7LL2

Cryo-EM structure of BG505 DS-SOSIP in complex with Glycan276-Dependent Broadly Neutralizing Antibody VRC33.01 Fab

7LL2 の概要

• エントリーDOI: 10.2210/pdb7ll2/pdb
• 関連するPDBエントリー: 7LL1 7LLK
• EMDBエントリー: 23412
• 分子名称: Envelope glycoprotein gp120, Envelope glycoprotein gp41, VRC33.01 Fab Heavy chain, ... (9 entities in total)
• 機能のキーワード: bg505 ds-sosip, glycan276-dependent, glycan276, vrc33.01 fab, hiv-1, immune system, immune system-viral protein complex, immune system/viral protein
• 由来する生物種: Human immunodeficiency virus 1; 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 379889.72

構造登録者

Manne, K.,Acharya, P. (登録日: 2021-02-03, 公開日: 2021-03-17, 最終更新日: 2024-10-30)

主引用文献

Cottrell, C.A.,Manne, K.,Kong, R.,Wang, S.,Zhou, T.,Chuang, G.Y.,Edwards, R.J.,Henderson, R.,Janowska, K.,Kopp, M.,Lin, B.C.,Louder, M.K.,Olia, A.S.,Rawi, R.,Shen, C.H.,Taft, J.D.,Torres, J.L.,Wu, N.R.,Zhang, B.,Doria-Rose, N.A.,Cohen, M.S.,Haynes, B.F.,Shapiro, L.,Ward, A.B.,Acharya, P.,Mascola, J.R.,Kwong, P.D.
Structural basis of glycan276-dependent recognition by HIV-1 broadly neutralizing antibodies.
Cell Rep, 37:109922-109922, 2021

PubMed Abstract: Recognition of N-linked glycan at residue N276 (glycan276) at the periphery of the CD4-binding site (CD4bs) on the HIV-envelope trimer is a formidable challenge for many CD4bs-directed antibodies. To understand how this glycan can be recognized, here we isolate two lineages of glycan276-dependent CD4bs antibodies. Antibody CH540-VRC40.01 (named for donor-lineage.clone) neutralizes 81% of a panel of 208 diverse strains, while antibody CH314-VRC33.01 neutralizes 45%. Cryo-electron microscopy (cryo-EM) structures of these two antibodies and 179NC75, a previously identified glycan276-dependent CD4bs antibody, in complex with HIV-envelope trimer reveal substantially different modes of glycan276 recognition. Despite these differences, binding of glycan276-dependent antibodies maintains a glycan276 conformation similar to that observed in the absence of glycan276-binding antibodies. By contrast, glycan276-independent CD4bs antibodies, such as VRC01, displace glycan276 upon binding. These results provide a foundation for understanding antibody recognition of glycan276 and suggest its presence may be crucial for priming immunogens seeking to initiate broad CD4bs recognition.

PubMed: 34731616
DOI: 10.1016/j.celrep.2021.109922

実験手法

ELECTRON MICROSCOPY (3.73 Å)