7LK9

Crystal structure of SARS-CoV-2 RBD-targeting antibody COV107-23 HC + COVD21-C8 LC

7LK9 の概要

• エントリーDOI: 10.2210/pdb7lk9/pdb
• 分子名称: COV107-23 heavy chain, COVD21-C8 light chain (2 entities in total)
• 機能のキーワード: coronavirus, covid-19, sars-cov-2, antibody, fab, receptor binding domain, immune system
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 186775.92

構造登録者

Yuan, M.,Zhu, X.,Wilson, I.A.,Wu, N.C. (登録日: 2021-02-01, 公開日: 2021-02-17, 最終更新日: 2023-10-18)

主引用文献

Tan, T.J.C.,Yuan, M.,Kuzelka, K.,Padron, G.C.,Beal, J.R.,Chen, X.,Wang, Y.,Rivera-Cardona, J.,Zhu, X.,Stadtmueller, B.M.,Brooke, C.B.,Wilson, I.A.,Wu, N.C.
Sequence signatures of two public antibody clonotypes that bind SARS-CoV-2 receptor binding domain.
Nat Commun, 12:3815-3815, 2021

PubMed Abstract: Since the COVID-19 pandemic onset, the antibody response to SARS-CoV-2 has been extensively characterized. Antibodies to the receptor binding domain (RBD) on the spike protein are frequently encoded by IGHV3-53/3-66 with a short complementarity-determining region (CDR) H3. Germline-encoded sequence motifs in heavy chain CDRs H1 and H2 have a major function, but whether any common motifs are present in CDR H3, which is often critical for binding specificity, is not clear. Here, we identify two public clonotypes of IGHV3-53/3-66 RBD antibodies with a 9-residue CDR H3 that pair with different light chains. Distinct sequence motifs on CDR H3 are present in the two public clonotypes that seem to be related to differential light chain pairing. Additionally, we show that Y58F is a common somatic hypermutation that results in increased binding affinity of IGHV3-53/3-66 RBD antibodies with a short CDR H3. These results advance understanding of the antibody response to SARS-CoV-2.

PubMed: 34155209
DOI: 10.1038/s41467-021-24123-7

実験手法

X-RAY DIFFRACTION (3.4 Å)