7LJY

Cryo-EM structure of the B dENE construct complexed with a 28-mer poly(A)

7LJY の概要

• エントリーDOI: 10.2210/pdb7ljy/pdb
• EMDBエントリー: 23401 23402
• 分子名称: B dENE construct, poly(A) (2 entities in total)
• 機能のキーワード: rna triple helix, rna stability, poly(a), saxs and cryo-em, rna
• 由来する生物種: Oryza sativa (rice); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 33414.99

構造登録者

Torabi, S.,Chen, Y.,Zhang, K.,Wang, J.,DeGregorio, S.,Vaidya, A.,Su, Z.,Pabit, S.,Chiu, W.,Pollack, L.,Steitz, J. (登録日: 2021-02-01, 公開日: 2021-04-14, 最終更新日: 2024-03-06)

主引用文献

Torabi, S.F.,Chen, Y.L.,Zhang, K.,Wang, J.,DeGregorio, S.J.,Vaidya, A.T.,Su, Z.,Pabit, S.A.,Chiu, W.,Pollack, L.,Steitz, J.A.
Structural analyses of an RNA stability element interacting with poly(A).
Proc.Natl.Acad.Sci.USA, 118:-, 2021

PubMed Abstract: Cis-acting RNA elements are crucial for the regulation of polyadenylated RNA stability. The element for nuclear expression (ENE) contains a U-rich internal loop flanked by short helices. An ENE stabilizes RNA by sequestering the poly(A) tail via formation of a triplex structure that inhibits a rapid deadenylation-dependent decay pathway. Structure-based bioinformatic studies identified numerous ENE-like elements in evolutionarily diverse genomes, including a subclass containing two ENE motifs separated by a short double-helical region (double ENEs [dENEs]). Here, the structure of a dENE derived from a rice transposable element (TWIFB1) before and after poly(A) binding (∼24 kDa and ∼33 kDa, respectively) is investigated. We combine biochemical structure probing, small angle X-ray scattering (SAXS), and cryo-electron microscopy (cryo-EM) to investigate the dENE structure and its local and global structural changes upon poly(A) binding. Our data reveal 1) the directionality of poly(A) binding to the dENE, and 2) that the dENE-poly(A) interaction involves a motif that protects the 3'-most seven adenylates of the poly(A). Furthermore, we demonstrate that the dENE does not undergo a dramatic global conformational change upon poly(A) binding. These findings are consistent with the recently solved crystal structure of a dENE+poly(A) complex [S.-F. Torabi , 371, eabe6523 (2021)]. Identification of additional modes of poly(A)-RNA interaction opens new venues for better understanding of poly(A) tail biology.

PubMed: 33785601
DOI: 10.1073/pnas.2026656118

実験手法

ELECTRON MICROSCOPY (5.6 Å)