7LCU

X-ray structure of Furin bound to BOS-318, a small molecule inhibitor

Summary for 7LCU

• Entry DOI: 10.2210/pdb7lcu/pdb
• Descriptor: Furin, CALCIUM ION, (1-{[2-(3,5-dichlorophenyl)-6-{[2-(4-methylpiperazin-1-yl)pyrimidin-5-yl]oxy}pyridin-4-yl]methyl}piperidin-4-yl)acetic acid, ... (5 entities in total)
• Functional Keywords: inhibitor protease, hydrolase-inhibitor complex, hydrolase/inhibitor
• Biological source: Homo sapiens (Human)
• Total number of polymer chains: 1
• Total formula weight: 54367.55

Authors

Campobasso, N.,Reid, R. (deposition date: 2021-01-11, release date: 2022-01-12, Last modification date: 2024-11-20)

Primary citation

Douglas, L.E.J.,Reihill, J.A.,Ho, M.W.Y.,Axten, J.M.,Campobasso, N.,Schneck, J.L.,Rendina, A.R.,Wilcoxen, K.M.,Martin, S.L.
A highly selective, cell-permeable furin inhibitor BOS-318 rescues key features of cystic fibrosis airway disease.
Cell Chem Biol, 29:947-957.e8, 2022

PubMed Abstract: In cystic fibrosis (CF), excessive furin activity plays a critical role in the activation of the epithelial sodium channel (ENaC), dysregulation of which contributes to airway dehydration, ineffective mucociliary clearance (MCC), and mucus obstruction. Here, we report a highly selective, cell-permeable furin inhibitor, BOS-318, that derives selectivity by eliciting the formation of a new, unexpected binding pocket independent of the active site catalytic triad. Using human ex vivo models, BOS-318 showed significant suppression of ENaC, which led to enhanced airway hydration and an ∼30-fold increase in MCC rate. Furin inhibition also protected ENaC from subsequent activation by neutrophil elastase, a soluble protease dominant in CF airways. Additional therapeutic benefits include protection against epithelial cell death induced by Pseudomonas aeruginosa exotoxin A. Our findings demonstrate the utility of selective furin inhibition as a mutation-agnostic approach that can correct features of CF airway pathophysiology in a manner expected to deliver therapeutic value.

PubMed: 35202587
DOI: 10.1016/j.chembiol.2022.02.001

Experimental method

X-RAY DIFFRACTION (1.24 Å)