7LCG

The mature Usutu SAAR-1776, Model A

7LCG の概要

• エントリーDOI: 10.2210/pdb7lcg/pdb
• EMDBエントリー: 23272 23273
• 分子名称: Envelope protein E, Membrane protein M, TRIMETHYL-[2-[[(2S,3S)-2-(OCTADECANOYLAMINO)-3-OXIDANYL-BUTOXY]-OXIDANYL-PHOSPHORYL]OXYETHYL]AZANIUM, ... (5 entities in total)
• 機能のキーワード: flavivirus, envelope glycoprotein, virus
• 由来する生物種: Usutu virus (USUV); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 191102.31

構造登録者

Khare, B.,Klose, T.,Fang, Q.,Kuhn, R. (登録日: 2021-01-11, 公開日: 2021-09-01, 最終更新日: 2024-10-30)

主引用文献

Khare, B.,Klose, T.,Fang, Q.,Rossmann, M.G.,Kuhn, R.J.
Structure of Usutu virus SAAR-1776 displays fusion loop asymmetry.
Proc.Natl.Acad.Sci.USA, 118:-, 2021

PubMed Abstract: Usutu virus (USUV) is an emerging arbovirus in Europe that has been increasingly identified in asymptomatic humans and donated blood samples and is a cause of increased incidents of neuroinvasive human disease. Treatment or prevention options for USUV disease are currently nonexistent, the result of a lack of understanding of the fundamental elements of USUV pathogenesis. Here, we report two structures of the mature USUV virus, determined at a resolution of 2.4 Å, using single-particle cryogenic electron microscopy. Mature USUV is an icosahedral shell of 180 copies of envelope (E) and membrane (M) proteins arranged in the classic herringbone pattern. However, unlike previous reports of flavivirus structures, we observe virus subpopulations and differences in the fusion loop disulfide bond. Presence of a second, unique E glycosylation site could elucidate host interactions, contributing to the broad USUV tissue tropism. The structures provide a basis for exploring USUV interactions with glycosaminoglycans and lectins, the role of the RGD motif as a receptor, and the inability of West Nile virus therapeutic antibody E16 to neutralize the mature USUV strain SAAR-1776. Finally, we identify three lipid binding sites and predict key residues that likely participate in virus stability and flexibility during membrane fusion. Our findings provide a framework for the development of USUV therapeutics and expand the current knowledge base of flavivirus biology.

PubMed: 34417300
DOI: 10.1073/pnas.2107408118

実験手法

ELECTRON MICROSCOPY (2.42 Å)