7L9C

Receiver Domain of RssB

7L9C の概要

• エントリーDOI: 10.2210/pdb7l9c/pdb
• 分子名称: Regulator of RpoS (2 entities in total)
• 機能のキーワード: receiver domain, response regulator, clpxp adaptor, signaling protein
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14566.07

構造登録者

Deaconescu, A.M.,Son, J.,Schwartz, J. (登録日: 2021-01-03, 公開日: 2021-04-07, 最終更新日: 2023-10-18)

主引用文献

Schwartz, J.,Son, J.,Brugger, C.,Deaconescu, A.M.
Phospho-dependent signaling during the general stress response by the atypical response regulator and ClpXP adaptor RssB.
Protein Sci., 30:899-907, 2021

PubMed Abstract: In the model organism Escherichia coli and related species, the general stress response relies on tight regulation of the intracellular levels of the promoter specificity subunit RpoS. RpoS turnover is exclusively dependent on RssB, a two-domain response regulator that functions as an adaptor that delivers RpoS to ClpXP for proteolysis. Here, we report crystal structures of the receiver domain of RssB both in its unphosphorylated form and bound to the phosphomimic BeF . Surprisingly, we find only modest differences between these two structures, suggesting that truncating RssB may partially activate the receiver domain to a "meta-active" state. Our structural and sequence analysis points to RssB proteins not conforming to either the Y-T coupling scheme for signaling seen in prototypical response regulators, such as CheY, or to the signaling model of the less understood FATGUY proteins.

PubMed: 33599047
DOI: 10.1002/pro.4047

実験手法

X-RAY DIFFRACTION (1.85 Å)