7L7V

Crystal structure of Arabidopsis NRG1.1 CC-R domain K94E/K96E/R99E/K100E/R103E/K106E/K110E mutant

7L7V の概要

• エントリーDOI: 10.2210/pdb7l7v/pdb
• 分子名称: Probable disease resistance protein At5g66900 (1 entity in total)
• 機能のキーワード: 4-helix bundle, cell death, membrane pore, calcium channel, immune system
• 由来する生物種: Arabidopsis thaliana (Mouse-ear cress)
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 95166.76

構造登録者

Walton, W.G.,Wan, L.,Lietzan, A.D.,Redinbo, M.R.,Dangl, J.L. (登録日: 2020-12-30, 公開日: 2021-06-16, 最終更新日: 2024-05-22)

主引用文献

Jacob, P.,Kim, N.H.,Wu, F.,El-Kasmi, F.,Chi, Y.,Walton, W.G.,Furzer, O.J.,Lietzan, A.D.,Sunil, S.,Kempthorn, K.,Redinbo, M.R.,Pei, Z.M.,Wan, L.,Dangl, J.L.
Plant "helper" immune receptors are Ca 2+ -permeable nonselective cation channels.
Science, 373:420-425, 2021

PubMed Abstract: Plant nucleotide-binding leucine-rich repeat receptors (NLRs) regulate immunity and cell death. In , a subfamily of "helper" NLRs is required by many "sensor" NLRs. Active NRG1.1 oligomerized, was enriched in plasma membrane puncta, and conferred cytoplasmic calcium ion (Ca) influx in plant and human cells. NRG1.1-dependent Ca influx and cell death were sensitive to Ca channel blockers and were suppressed by mutations affecting oligomerization or plasma membrane enrichment. Ca influx and cell death mediated by NRG1.1 and ACTIVATED DISEASE RESISTANCE 1 (ADR1), another helper NLR, required conserved negatively charged N-terminal residues. Whole-cell voltage-clamp recordings demonstrated that helper NLRs form Ca-permeable cation channels to directly regulate cytoplasmic Ca levels and consequent cell death. Thus, helper NLRs transduce cell death signals directly.

PubMed: 34140391
DOI: 10.1126/science.abg7917

実験手法

X-RAY DIFFRACTION (2.95 Å)