7L5A

Crystal structure of the photosensory module from Xanthomonas campestris bacteriophytochrome XccBphP in the Pfr state

7L5A の概要

• エントリーDOI: 10.2210/pdb7l5a/pdb
• 分子名称: Bacteriophytochrome, BILIVERDINE IX ALPHA (3 entities in total)
• 機能のキーワード: signaling protein, photoreceptor, bacterial protein, photosensor, red/far-red light, phytochrome, signal transduction, phytopathogen
• 由来する生物種: Xanthomonas campestris pv. campestris (strain 8004)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 58355.61

構造登録者

Otero, L.H.,Antelo, G.,Sanchez-Lamas, M.,Klinke, S.,Goldbaum, F.A.,Rinaldi, J.,Bonomi, H.R. (登録日: 2020-12-21, 公開日: 2021-12-29, 最終更新日: 2024-11-20)

主引用文献

Otero, L.H.,Foscaldi, S.,Antelo, G.T.,Rosano, G.L.,Sirigu, S.,Klinke, S.,Defelipe, L.A.,Sanchez-Lamas, M.,Battocchio, G.,Conforte, V.,Vojnov, A.A.,Chavas, L.M.G.,Goldbaum, F.A.,Mroginski, M.A.,Rinaldi, J.,Bonomi, H.R.
Structural basis for the Pr-Pfr long-range signaling mechanism of a full-length bacterial phytochrome at the atomic level.
Sci Adv, 7:eabh1097-eabh1097, 2021

PubMed Abstract: Phytochromes constitute a widespread photoreceptor family that typically interconverts between two photostates called Pr (red light–absorbing) and Pfr (far-red light–absorbing). The lack of full-length structures solved at the (near-)atomic level in both pure Pr and Pfr states leaves gaps in the structural mechanisms involved in the signal transmission pathways during the photoconversion. Here, we present the crystallographic structures of three versions from the plant pathogen virulence regulator BphP bacteriophytochrome, including two full-length proteins, in the Pr and Pfr states. The structures show a reorganization of the interaction networks within and around the chromophore-binding pocket, an α-helix/β-sheet tongue transition, and specific domain reorientations, along with interchanging kinks and breaks at the helical spine as a result of the photoswitching, which subsequently affect the quaternary assembly. These structural findings, combined with multidisciplinary studies, allow us to describe the signaling mechanism of a full-length bacterial phytochrome at the atomic level.

PubMed: 34818032
DOI: 10.1126/sciadv.abh1097

実験手法

X-RAY DIFFRACTION (2.95 Å)