7L2E

Cryo-EM structure of NTD-directed neutralizing antibody 4-18 in complex with prefusion SARS-CoV-2 spike glycoprotein

7L2E の概要

• エントリーDOI: 10.2210/pdb7l2e/pdb
• EMDBエントリー: 23126
• 分子名称: Spike glycoprotein, 4-18 heavy chain, 4-18 light chain, ... (5 entities in total)
• 機能のキーワード: neutralizing antibody, fusion protein, spike glycoprotein, covid-19, ntd, ntd-directed antibody, 4-18, viral protein, immune system, viral protein-immune system complex, viral protein/immune system
• 由来する生物種: Severe acute respiratory syndrome coronavirus 2 (2019-nCoV); 詳細
• タンパク質・核酸の鎖数: 9
• 化学式量合計: 586621.15

構造登録者

Cerutti, G.,Shapiro, L. (登録日: 2020-12-16, 公開日: 2021-03-24, 最終更新日: 2024-11-06)

主引用文献

Cerutti, G.,Guo, Y.,Zhou, T.,Gorman, J.,Lee, M.,Rapp, M.,Reddem, E.R.,Yu, J.,Bahna, F.,Bimela, J.,Huang, Y.,Katsamba, P.S.,Liu, L.,Nair, M.S.,Rawi, R.,Olia, A.S.,Wang, P.,Zhang, B.,Chuang, G.Y.,Ho, D.D.,Sheng, Z.,Kwong, P.D.,Shapiro, L.
Potent SARS-CoV-2 neutralizing antibodies directed against spike N-terminal domain target a single supersite.
Cell Host Microbe, 29:819-, 2021

PubMed Abstract: Numerous antibodies that neutralize SARS-CoV-2 have been identified, and these generally target either the receptor-binding domain (RBD) or the N-terminal domain (NTD) of the viral spike. While RBD-directed antibodies have been extensively studied, far less is known about NTD-directed antibodies. Here, we report cryo-EM and crystal structures for seven potent NTD-directed neutralizing antibodies in complex with spike or isolated NTD. These structures defined several antibody classes, with at least one observed in multiple convalescent donors. The structures revealed that all seven antibodies target a common surface, bordered by glycans N17, N74, N122, and N149. This site-formed primarily by a mobile β-hairpin and several flexible loops-was highly electropositive, located at the periphery of the spike, and the largest glycan-free surface of NTD facing away from the viral membrane. Thus, in contrast to neutralizing RBD-directed antibodies that recognize multiple non-overlapping epitopes, potent NTD-directed neutralizing antibodies appear to target a single supersite.

PubMed: 33789084
DOI: 10.1016/j.chom.2021.03.005

実験手法

ELECTRON MICROSCOPY (2.97 Å)