Loading
PDBj
メニューPDBj@FacebookPDBj@X(formerly Twitter)PDBj@BlueSkyPDBj@YouTubewwPDB FoundationwwPDBDonate
RCSB PDBPDBeBMRBAdv. SearchSearch help

7KWM

CtBP1 (28-375) L182F/V185T - AMP

7KWM の概要
エントリーDOI10.2210/pdb7kwm/pdb
関連するPDBエントリー6V89 6V8A
分子名称C-terminal-binding protein 1, ADENOSINE MONOPHOSPHATE, CALCIUM ION, ... (4 entities in total)
機能のキーワードc-terminal binding protein, co-transcriptional factor, d-isomer specific 2-hydroxyacid dehydrogenase, transcription
由来する生物種Homo sapiens (Human)
タンパク質・核酸の鎖数1
化学式量合計38929.14
構造登録者
Royer, W.E.,Del Campo, M. (登録日: 2020-12-01, 公開日: 2021-02-03, 最終更新日: 2023-10-18)
主引用文献Nichols, J.C.,Schiffer, C.A.,Royer Jr., W.E.
NAD(H) phosphates mediate tetramer assembly of human C-terminal binding protein (CtBP).
J.Biol.Chem., 296:100351-100351, 2021
Cited by
PubMed Abstract: C-terminal binding proteins (CtBPs) are cotranscriptional factors that play key roles in cell fate. We have previously shown that NAD(H) promotes the assembly of similar tetramers from either human CtBP1 and CtBP2 and that CtBP2 tetramer destabilizing mutants are defective for oncogenic activity. To assist structure-based design efforts for compounds that disrupt CtBP tetramerization, it is essential to understand how NAD(H) triggers tetramer assembly. Here, we investigate the moieties within NAD(H) that are responsible for triggering tetramer formation. Using multiangle light scattering (MALS), we show that ADP is able to promote tetramer formation of both CtBP1 and CtBP2, whereas AMP promotes tetramer assembly of CtBP1, but not CtBP2. Other NAD(H) moieties that lack the adenosine phosphate, including adenosine and those incorporating nicotinamide, all fail to promote tetramer assembly. Our crystal structures of CtBP1 with AMP reveal participation of the adenosine phosphate in the tetrameric interface, pinpointing its central role in NAD(H)-linked assembly. CtBP1 and CtBP2 have overlapping but unique roles, suggesting that a detailed understanding of their unique structural properties might have utility in the design of paralog-specific inhibitors. We investigated the different responses to AMP through a series of site-directed mutants at 13 positions. These mutations reveal a central role for a hinge segment, which we term the 120s hinge that connects the substrate with coenzyme-binding domains and influences nucleotide binding and tetramer assembly. Our results provide insight into suitable pockets to explore in structure-based drug design to interfere with cotranscriptional activity of CtBP in cancer.
PubMed: 33524397
DOI: 10.1016/j.jbc.2021.100351
主引用文献が同じPDBエントリー
実験手法
X-RAY DIFFRACTION (2.3 Å)
構造検証レポート
Validation report summary of 7kwm
検証レポート(詳細版)ダウンロードをダウンロード

229380

件を2024-12-25に公開中

PDB statisticsPDBj update infoContact PDBjnumon