7KTR

Cryo-EM structure of the human SAGA coactivator complex (TRRAP, core)

7KTR の概要

• エントリーDOI: 10.2210/pdb7ktr/pdb
• 関連するPDBエントリー: 7KTS
• EMDBエントリー: 23027 23028
• 分子名称: Transformation/transcription domain-associated protein,Transformation/transcription domain-associated protein,Transformation/transcription domain-associated protein,Transformation/transcription domain-associated protein, Transcriptional adapter 1, Ataxin-7, ... (12 entities in total)
• 機能のキーワード: coactivator, gene regulation, transcription, chromatin
• 由来する生物種: Homo sapiens; 詳細
• タンパク質・核酸の鎖数: 11
• 化学式量合計: 887727.54

構造登録者

Herbst, D.A.,Esbin, M.N.,Nogales, E. (登録日: 2020-11-24, 公開日: 2021-11-10, 最終更新日: 2024-05-29)

主引用文献

Herbst, D.A.,Esbin, M.N.,Louder, R.K.,Dugast-Darzacq, C.,Dailey, G.M.,Fang, Q.,Darzacq, X.,Tjian, R.,Nogales, E.
Structure of the human SAGA coactivator complex.
Nat.Struct.Mol.Biol., 28:989-996, 2021

PubMed Abstract: The SAGA complex is a regulatory hub involved in gene regulation, chromatin modification, DNA damage repair and signaling. While structures of yeast SAGA (ySAGA) have been reported, there are noteworthy functional and compositional differences for this complex in metazoans. Here we present the cryogenic-electron microscopy (cryo-EM) structure of human SAGA (hSAGA) and show how the arrangement of distinct structural elements results in a globally divergent organization from that of yeast, with a different interface tethering the core module to the TRRAP subunit, resulting in a dramatically altered geometry of functional elements and with the integration of a metazoan-specific splicing module. Our hSAGA structure reveals the presence of an inositol hexakisphosphate (InsP) binding site in TRRAP and an unusual property of its pseudo-(Ψ)PIKK. Finally, we map human disease mutations, thus providing the needed framework for structure-guided drug design of this important therapeutic target for human developmental diseases and cancer.

PubMed: 34811519
DOI: 10.1038/s41594-021-00682-7

実験手法

ELECTRON MICROSCOPY (2.93 Å)