7KSF

Crystal structure of Prototype Foamy Virus Protease-Reverse Transcriptase (native)

Summary for 7KSF

• Entry DOI: 10.2210/pdb7ksf/pdb
• Descriptor: Protease/Reverse transcriptase/ribonuclease H, CALCIUM ION (3 entities in total)
• Functional Keywords: aspartyl protease, hydrolase, multifunctional enzyme, transferase, reverse transcription, viral maturation, viral protein
• Biological source: Eastern chimpanzee simian foamy virus
• Total number of polymer chains: 1
• Total formula weight: 85213.11

Authors

Harrison, J.J.E.K.,Das, K.,Ruiz, F.X.,Arnold, E. (deposition date: 2020-11-21, release date: 2021-08-11, Last modification date: 2023-10-18)

Primary citation

Harrison, J.J.E.K.,Tuske, S.,Das, K.,Ruiz, F.X.,Bauman, J.D.,Boyer, P.L.,DeStefano, J.J.,Hughes, S.H.,Arnold, E.
Crystal Structure of a Retroviral Polyprotein: Prototype Foamy Virus Protease-Reverse Transcriptase (PR-RT).
Viruses, 13:-, 2021

PubMed Abstract: In most cases, proteolytic processing of the retroviral Pol portion of the Gag-Pol polyprotein precursor produces protease (PR), reverse transcriptase (RT), and integrase (IN). However, foamy viruses (FVs) express Pol separately from Gag and, when Pol is processed, only the IN domain is released. Here, we report a 2.9 Å resolution crystal structure of the mature PR-RT from prototype FV (PFV) that can carry out both proteolytic processing and reverse transcription but is in a configuration not competent for proteolytic or polymerase activity. PFV PR-RT is monomeric and the architecture of PFV PR is similar to one of the subunits of HIV-1 PR, which is a dimer. There is a C-terminal extension of PFV PR (101-145) that consists of two helices which are adjacent to the base of the RT palm subdomain, and anchors PR to RT. The polymerase domain of PFV RT consists of fingers, palm, thumb, and connection subdomains whose spatial arrangements are similar to the p51 subunit of HIV-1 RT. The RNase H and polymerase domains of PFV RT are connected by flexible linkers. Significant spatial and conformational (sub)domain rearrangements are therefore required for nucleic acid binding. The structure of PFV PR-RT provides insights into the conformational maturation of retroviral Pol polyproteins.

PubMed: 34452360
DOI: 10.3390/v13081495

Experimental method

X-RAY DIFFRACTION (2.9 Å)