7KPD

NMR Solution Structure of Acyclotide ribe 31

7KPD の概要

• エントリーDOI: 10.2210/pdb7kpd/pdb
• NMR情報: BMRB: 30813
• 分子名称: Acyclotide ribe 31 (1 entity in total)
• 機能のキーワード: antimicrobial protein
• 由来する生物種: Rinorea bengalensis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 3139.80

構造登録者

Harvey, P.J.,Craik, D.J.,Dang, T.T. (登録日: 2020-11-11, 公開日: 2021-02-24, 最終更新日: 2024-11-20)

主引用文献

Dang, T.T.,Chan, L.Y.,Tombling, B.J.,Harvey, P.J.,Gilding, E.K.,Craik, D.J.
In Planta Discovery and Chemical Synthesis of Bracelet Cystine Knot Peptides from Rinorea bengalensis .
J.Nat.Prod., 2021

PubMed Abstract: Cyclotides are plant-derived peptides that have attracted interest as biocides and scaffolds for the development of stable peptide therapeutics. Cyclotides are characterized by their cyclic backbone and cystine knot framework, which engenders them with remarkably high stability. This study reports the cystine knot-related peptidome of , a small rainforest tree in the Violaceae family that is distributed from Australia westward to India. Surprisingly, many more acyclic knotted peptides (acyclotides) were discovered than cyclic counterparts (cyclotides), with 32 acyclotides and 1 cyclotide sequenced using combined transcriptome and proteomic analyses. Nine acyclotides were isolated and screened against a panel of mammalian cell lines, showing they had the cytotoxic properties normally associated with cyclotide-like peptides. NMR analysis of the acyclotide ribes 21 and 22 and the cyclotide ribe 33 confirmed that these peptides contained the cystine knot structural motif. The bracelet-subfamily cyclotide ribe 33 was amenable to chemical synthesis in reasonable yield, an achievement that has long eluded previous attempts to synthetically produce bracelet cyclotides. Accordingly, ribe 33 represents an exciting new bracelet cyclotide scaffold that can be subject to chemical modification for future molecular engineering applications.

PubMed: 33570395
DOI: 10.1021/acs.jnatprod.0c01065

実験手法

SOLUTION NMR