7KJT

KEOPS tRNA modifying sub-complex of archaeal Cgi121 and tRNA

7KJT の概要

• エントリーDOI: 10.2210/pdb7kjt/pdb
• 関連するPDBエントリー: 7KJU
• 分子名称: RNA (70-MER), Regulatory protein Cgi121 (2 entities in total)
• 機能のキーワード: keops, trna, cgi121, rna modifying enzyme, n6-threonylcarbamoyl adenosine, rna binding protein-rna complex, rna binding protein/rna
• 由来する生物種: Methanocaldococcus jannaschii; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 41850.74

構造登録者

Ceccarelli, D.F.,Beenstock, J.,Mao, D.Y.L.,Sicheri, F. (登録日: 2020-10-26, 公開日: 2020-12-02, 最終更新日: 2025-06-25)

主引用文献

Beenstock, J.,Ona, S.M.,Porat, J.,Orlicky, S.,Wan, L.C.K.,Ceccarelli, D.F.,Maisonneuve, P.,Szilard, R.K.,Yin, Z.,Setiaputra, D.,Mao, D.Y.L.,Khan, M.,Raval, S.,Schriemer, D.C.,Bayfield, M.A.,Durocher, D.,Sicheri, F.
A substrate binding model for the KEOPS tRNA modifying complex.
Nat Commun, 11:6233-6233, 2020

PubMed Abstract: The KEOPS complex, which is conserved across archaea and eukaryotes, is composed of four core subunits; Pcc1, Kae1, Bud32 and Cgi121. KEOPS is crucial for the fitness of all organisms examined. In humans, pathogenic mutations in KEOPS genes lead to Galloway-Mowat syndrome, an autosomal-recessive disease causing childhood lethality. Kae1 catalyzes the universal and essential tRNA modification N-threonylcarbamoyl adenosine, but the precise roles of all other KEOPS subunits remain an enigma. Here we show using structure-guided studies that Cgi121 recruits tRNA to KEOPS by binding to its 3' CCA tail. A composite model of KEOPS bound to tRNA reveals that all KEOPS subunits form an extended tRNA-binding surface that we have validated in vitro and in vivo to mediate the interaction with the tRNA substrate and its modification. These findings provide a framework for understanding the inner workings of KEOPS and delineate why all KEOPS subunits are essential.

PubMed: 33277478
DOI: 10.1038/s41467-020-19990-5

実験手法

X-RAY DIFFRACTION (3.34 Å)