7KH5

Hen Egg White Lysozyme in complex with tetrabromoterephthalic acid

7KH5 の概要

• エントリーDOI: 10.2210/pdb7kh5/pdb
• 分子名称: Lysozyme C, 2,3,5,6-tetrabromobenzene-1,4-dicarboxylic acid (3 entities in total)
• 機能のキーワード: lyzozyme, hydrolase, antimicrobial protein
• 由来する生物種: Gallus gallus (Chicken)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14812.88

構造登録者

Truong, J.,Nguyen, S. (登録日: 2020-10-20, 公開日: 2021-04-28, 最終更新日: 2021-05-19)

主引用文献

Truong, J.Q.,Nguyen, S.,Bruning, J.B.,Shearwin, K.E.
Simplified heavy-atom derivatization of protein structures via co-crystallization with the MAD tetragon tetrabromoterephthalic acid.
Acta Crystallogr.,Sect.F, 77:156-162, 2021

PubMed Abstract: The phase problem is a persistent bottleneck that impedes the structure-determination pipeline and must be solved to obtain atomic resolution crystal structures of macromolecules. Although molecular replacement has become the predominant method of solving the phase problem, many scenarios still exist in which experimental phasing is needed. Here, a proof-of-concept study is presented that shows the efficacy of using tetrabromoterephthalic acid (B4C) as an experimental phasing compound. Incorporating B4C into the crystal lattice using co-crystallization, the crystal structure of hen egg-white lysozyme was solved using MAD phasing. The strong anomalous signal generated by its four Br atoms coupled with its compatibility with commonly used crystallization reagents render B4C an effective experimental phasing compound that can be used to overcome the phase problem.

PubMed: 33949976
DOI: 10.1107/S2053230X21004052

実験手法

X-RAY DIFFRACTION (1.295 Å)