7KFR

Adeno-Associated Virus (AAV-DJ) - cryo-EM structure at 1.56 Angstrom Resolution

7KFR の概要

• エントリーDOI: 10.2210/pdb7kfr/pdb
• EMDBエントリー: 22854
• 分子名称: Capsid protein VP1, MAGNESIUM ION (3 entities in total)
• 機能のキーワード: gene therapy vector, virus like particle, virus
• 由来する生物種: Adeno-associated virus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 58950.72

構造登録者

Xie, Q.,Yoshioka, C.K.,Chapman, M.S. (登録日: 2020-10-14, 公開日: 2020-12-16, 最終更新日: 2024-03-06)

主引用文献

Xie, Q.,Yoshioka, C.K.,Chapman, M.S.
Adeno-Associated Virus (AAV-DJ)-Cryo-EM Structure at 1.56 Angstroms Resolution.
Viruses, 12:-, 2020

PubMed Abstract: Adeno-associated virus is the leading viral vector for gene therapy. AAV-DJ is a recombinant variant developed for tropism to the liver. The AAV-DJ structure has been determined to 1.56 Å resolution through cryo-electron microscopy (cryo-EM). Only apoferritin is reported in preprints at 1.6 Å or higher resolution, and AAV-DJ nearly matches the highest resolutions ever attained through X-ray diffraction of virus crystals. However, cryo-EM has the advantage that most of the hydrogens are clear, improving the accuracy of atomic refinement, and removing ambiguity in hydrogen bond identification. Outside of secondary structures where hydrogen bonding was predictable a priori, the networks of hydrogen bonds coming from direct observation of hydrogens and acceptor atoms are quite different from those inferred even at 2.8 Å resolution. The implications for understanding viral assembly mean that cryo-EM will likely become the favored approach for high resolution structural virology.

PubMed: 33092282
DOI: 10.3390/v12101194

実験手法

ELECTRON MICROSCOPY (1.56 Å)