7KBB

Cryo-EM structure of the HCMV pentamer bound by Fabs 2-18 and 8I21

7KBB の概要

• エントリーDOI: 10.2210/pdb7kbb/pdb
• EMDBエントリー: 22788
• 分子名称: 2-18 Fab Heavy Chain, 2-18 Fab Light Chain, Envelope protein UL128, ... (7 entities in total)
• 機能のキーワード: hcmv pentamer, fab, complex, viral protein, viral protein-immune system complex, viral protein/immune system
• 由来する生物種: Homo sapiens; 詳細
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 149161.34

構造登録者

Wrapp, D.,McLellan, J.S. (登録日: 2020-10-01, 公開日: 2021-08-11, 最終更新日: 2024-10-23)

主引用文献

Wrapp, D.,Ye, X.,Ku, Z.,Su, H.,Jones, H.G.,Wang, N.,Mishra, A.K.,Freed, D.C.,Li, F.,Tang, A.,Li, L.,Jaijyan, D.K.,Zhu, H.,Wang, D.,Fu, T.M.,Zhang, N.,An, Z.,McLellan, J.S.
Structural basis for HCMV Pentamer recognition by neuropilin 2 and neutralizing antibodies.
Sci Adv, 8:eabm2546-eabm2546, 2022

PubMed Abstract: Human cytomegalovirus (HCMV) encodes multiple surface glycoprotein complexes to infect a variety of cell types. The HCMV Pentamer, composed of gH, gL, UL128, UL130, and UL131A, enhances entry into epithelial, endothelial, and myeloid cells by interacting with the cell surface receptor neuropilin 2 (NRP2). Despite the critical nature of this interaction, the molecular determinants that govern NRP2 recognition remain unclear. Here, we describe the cryo-EM structure of NRP2 bound to Pentamer. The high-affinity interaction between these proteins is calcium dependent and differs from the canonical carboxyl-terminal arginine (CendR) binding that NRP2 typically uses. We also determine the structures of four neutralizing human antibodies bound to the HCMV Pentamer to define susceptible epitopes. Two of these antibodies compete with NRP2 binding, but the two most potent antibodies recognize a previously unidentified epitope that does not overlap the NRP2-binding site. Collectively, these findings provide a structural basis for HCMV tropism and antibody-mediated neutralization.

PubMed: 35275718
DOI: 10.1126/sciadv.abm2546

実験手法

ELECTRON MICROSCOPY (4.02 Å)