7K5T

Bst DNA polymerase I time-resolved structure, 25.5 hr post dATP and dCTP addition

7K5T の概要

• エントリーDOI: 10.2210/pdb7k5t/pdb
• 分子名称: DNA (5'-D(P*CP*GP*AP*TP*CP*AP*CP*GP*TP*A)-3'), DNA (5'-D(P*CP*GP*TP*AP*CP*GP*TP*GP*AP*TP*CP*GP*C)-3'), DNA polymerase I, ... (6 entities in total)
• 機能のキーワード: dna polymerase, transferase, transferase-dna complex, transferase/dna
• 由来する生物種: Geobacillus stearothermophilus; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 75270.70

構造登録者

Chim, N.,Meza, R.A.,Trinh, A.M.,Chaput, J.C. (登録日: 2020-09-17, 公開日: 2021-03-03, 最終更新日: 2023-10-18)

主引用文献

Chim, N.,Meza, R.A.,Trinh, A.M.,Yang, K.,Chaput, J.C.
Following replicative DNA synthesis by time-resolved X-ray crystallography.
Nat Commun, 12:2641-2641, 2021

PubMed Abstract: The mechanism of DNA synthesis has been inferred from static structures, but the absence of temporal information raises longstanding questions about the order of events in one of life's most central processes. Here we follow the reaction pathway of a replicative DNA polymerase using time-resolved X-ray crystallography to elucidate the order and transition between intermediates. In contrast to the canonical model, the structural changes observed in the time-lapsed images reveal a catalytic cycle in which translocation precedes catalysis. The translocation step appears to follow a push-pull mechanism where the O-O1 loop of the finger subdomain acts as a pawl to facilitate unidirectional movement along the template with conserved tyrosine residues 714 and 719 functioning as tandem gatekeepers of DNA synthesis. The structures capture the precise order of critical events that may be a general feature of enzymatic catalysis among replicative DNA polymerases.

PubMed: 33976175
DOI: 10.1038/s41467-021-22937-z

実験手法

X-RAY DIFFRACTION (2.3 Å)