7JZJ

Crystal structure demonstrating CTD-CTD interactions of Zaire Ebola virus VP40 dimer

7JZJ の概要

• エントリーDOI: 10.2210/pdb7jzj/pdb
• 分子名称: Matrix protein VP40, PENTAETHYLENE GLYCOL (3 entities in total)
• 機能のキーワード: vp40, matrix protein, ebola virus, viral protein
• 由来する生物種: Zaire ebolavirus (strain Mayinga-76) (ZEBOV)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 129631.37

構造登録者

Norris, M.J.,Bornholdt, Z.A.,Saphire, E.O. (登録日: 2020-09-02, 公開日: 2020-10-28, 最終更新日: 2024-10-23)

主引用文献

Wan, W.,Clarke, M.,Norris, M.J.,Kolesnikova, L.,Koehler, A.,Bornholdt, Z.A.,Becker, S.,Ollman-Saphire, E.,Briggs, J.A.
Ebola and Marburg virus matrix layers are locally ordered assemblies of VP40 dimers.
Elife, 9:-, 2020

PubMed Abstract: Filoviruses such as Ebola and Marburg virus bud from the host membrane as enveloped virions. This process is achieved by the matrix protein VP40. When expressed alone, VP40 induces budding of filamentous virus-like particles, suggesting that localization to the plasma membrane, oligomerization into a matrix layer, and generation of membrane curvature are intrinsic properties of VP40. There has been no direct information on the structure of VP40 matrix layers within viruses or virus-like particles. We present structures of Ebola and Marburg VP40 matrix layers in intact virus-like particles, and within intact Marburg viruses. VP40 dimers assemble extended chains via C-terminal domain interactions. These chains stack to form 2D matrix lattices below the membrane surface. These lattices form a patchwork assembly across the membrane and suggesting that assembly may begin at multiple points. Our observations define the structure and arrangement of the matrix protein layer that mediates formation of filovirus particles.

PubMed: 33016878
DOI: 10.7554/eLife.59225

実験手法

X-RAY DIFFRACTION (2.46 Å)