7JXF

E. coli TSase complex with a bi-substrate reaction intermediate diastereomer analog

7JXF の概要

• エントリーDOI: 10.2210/pdb7jxf/pdb
• 分子名称: Thymidylate synthase, 5-HYDROXYMETHYLURIDINE-2'-DEOXY-5'-MONOPHOSPHATE, N-[4-({[(6S)-2,4-diamino-5,6,7,8-tetrahydropyrido[3,2-d]pyrimidin-6-yl]methyl}amino)benzene-1-carbonyl]-L-glutamic acid, ... (6 entities in total)
• 機能のキーワード: non covalent intermediate, half-sites activity, tmp synthesis, inhibitor, biosynthetic protein
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 62733.61

構造登録者

Finer-Moore, J.,Kholodar, S.A.,Stroud, R.M.,Kohen, A.,Moliner, V.,Swiderek, K. (登録日: 2020-08-27, 公開日: 2021-04-21, 最終更新日: 2024-11-20)

主引用文献

Kholodar, S.A.,Finer-Moore, J.S.,Swiderek, K.,Arafet, K.,Moliner, V.,Stroud, R.M.,Kohen, A.
Caught in Action: X-ray Structure of Thymidylate Synthase with Noncovalent Intermediate Analog.
Biochemistry, 60:1243-1247, 2021

PubMed Abstract: Methylation of 2-deoxyuridine-5'-monophosphate (dUMP) at the C5 position by the obligate dimeric thymidylate synthase (TSase) in the sole biosynthetic pathway to thymidine 5'-monophosphate (dTMP) proceeds by forming a covalent ternary complex with dUMP and cosubstrate 5,10-methylenetetrahydrofolate. The crystal structure of an analog of this intermediate gives important mechanistic insights but does not explain the half-of-the-sites activity of the enzyme. Recent experiments showed that the C5 proton and the catalytic Cys are eliminated in a concerted manner from the covalent ternary complex to produce a noncovalent bisubstrate intermediate. Here, we report the crystal structure of TSase with a close synthetic analog of this intermediate in which it has partially reacted with the enzyme but in only one protomer, consistent with the half-of-the-sites activity of this enzyme. Quantum mechanics/molecular mechanics simulations confirmed that the analog could undergo catalysis. The crystal structure shows a new water 2.9 Å from the critical C5 of the dUMP moiety, which in conjunction with other residues in the network, may be the elusive general base that abstracts the C5 proton of dUMP during the reaction.

PubMed: 33829766
DOI: 10.1021/acs.biochem.1c00063

実験手法

X-RAY DIFFRACTION (1.5 Å)