7JU4

Radial spoke 2 stalk, IDAc, and N-DRC attached with doublet microtubule

This is a non-PDB format compatible entry.

Summary for 7JU4

• Entry DOI: 10.2210/pdb7ju4/pdb
• EMDB information: 22475 22480 22481 22482 22483 22486
• Descriptor: Dynein regulatory complex subunit 4, Radial spoke protein 7, Radial spoke protein 11, ... (23 entities in total)
• Functional Keywords: cilia, native, complex, microtubule, mechanoregulation, structural protein
• Biological source: Chlamydomonas reinhardtii; More
• Total number of polymer chains: 61
• Total formula weight: 2675852.28

Authors

Gui, M.,Ma, M.,Sze-Tu, E.,Wang, X.,Koh, F.,Zhong, E.,Berger, B.,Davis, J.,Dutcher, S.,Zhang, R.,Brown, A. (deposition date: 2020-08-19, release date: 2020-12-16, Last modification date: 2024-03-06)

Primary citation

Gui, M.,Ma, M.,Sze-Tu, E.,Wang, X.,Koh, F.,Zhong, E.D.,Berger, B.,Davis, J.H.,Dutcher, S.K.,Zhang, R.,Brown, A.
Structures of radial spokes and associated complexes important for ciliary motility.
Nat.Struct.Mol.Biol., 28:29-37, 2021

PubMed Abstract: In motile cilia, a mechanoregulatory network is responsible for converting the action of thousands of dynein motors bound to doublet microtubules into a single propulsive waveform. Here, we use two complementary cryo-EM strategies to determine structures of the major mechanoregulators that bind ciliary doublet microtubules in Chlamydomonas reinhardtii. We determine structures of isolated radial spoke RS1 and the microtubule-bound RS1, RS2 and the nexin-dynein regulatory complex (N-DRC). From these structures, we identify and build atomic models for 30 proteins, including 23 radial-spoke subunits. We reveal how mechanoregulatory complexes dock to doublet microtubules with regular 96-nm periodicity and communicate with one another. Additionally, we observe a direct and dynamically coupled association between RS2 and the dynein motor inner dynein arm subform c (IDAc), providing a molecular basis for the control of motor activity by mechanical signals. These structures advance our understanding of the role of mechanoregulation in defining the ciliary waveform.

PubMed: 33318703
DOI: 10.1038/s41594-020-00530-0

Experimental method

ELECTRON MICROSCOPY (3.4 Å)