7JSV

Cryo-EM structure of conjugative pili from carbapenem-resistant Klebsiella pneumoniae

7JSV の概要

• エントリーDOI: 10.2210/pdb7jsv/pdb
• EMDBエントリー: 22460
• 分子名称: Pilin, 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE (2 entities in total)
• 機能のキーワード: conjugation pili, helical reconstruction, protein fibril
• 由来する生物種: Klebsiella pneumoniae
• タンパク質・核酸の鎖数: 41
• 化学式量合計: 329952.95

構造登録者

Zheng, W.,Pena, A.,Frankel, G.,Egelman, E.H. (登録日: 2020-08-16, 公開日: 2020-09-02, 最終更新日: 2024-03-06)

主引用文献

Zheng, W.,Pena, A.,Low, W.W.,Wong, J.L.C.,Frankel, G.,Egelman, E.H.
Cryoelectron-Microscopic Structure of the pKpQIL Conjugative Pili from Carbapenem-Resistant Klebsiella pneumoniae.
Structure, 28:1321-, 2020

PubMed Abstract: Conjugative pili are important in mediating bacterial conjugation and horizontal gene transfer. Since plasmid transfer can include antibiotic-resistance genes, conjugation is an important mechanism in the spread of antibiotic resistance. Filamentous bacteriophages have been shown to exist in two different structural classes: those with a 5-fold rotational symmetry and those with a one-start helix with approximately 5 subunits per turn. Structures for the F and the F-like pED208 conjugation pilus have shown that they have 5-fold rotational symmetry. Here, we report the cryoelectron-microscopic structure of conjugative pili from carbapenem-resistant Klebsiella pneumoniae, encoded on the IncFIIK pKpQIL plasmid, at 3.9 Å resolution and show that it has a one-start helix. These results establish that conjugation pili can exist in at least two structural classes, consistent with other results showing that relatively small perturbations are needed to change the helical symmetry of polymers.

PubMed: 32916103
DOI: 10.1016/j.str.2020.08.010

実験手法

ELECTRON MICROSCOPY (3.9 Å)