7JRR

Crystal structures of artificially designed homomeric RNA nanoarchitectures

Summary for 7JRR

• Entry DOI: 10.2210/pdb7jrr/pdb
• Descriptor: RNA (50-MER), MAGNESIUM ION, MANGANESE (II) ION, ... (4 entities in total)
• Functional Keywords: nano structure, dimeric parallelogram, rna
• Biological source: synthetic construct
• Total number of polymer chains: 1
• Total formula weight: 17073.12

Authors

Liu, D.,Shao, Y.,Piccirilli, J.A.,Weizmann, Y. (deposition date: 2020-08-12, release date: 2021-09-08, Last modification date: 2023-10-18)

Primary citation

Liu, D.,Shao, Y.,Piccirilli, J.A.,Weizmann, Y.
Structures of artificially designed discrete RNA nanoarchitectures at near-atomic resolution.
Sci Adv, 7:eabf4459-eabf4459, 2021

PubMed Abstract: Although advances in nanotechnology have enabled the construction of complex and functional synthetic nucleic acid–based nanoarchitectures, high-resolution discrete structures are lacking because of the difficulty in obtaining good diffracting crystals. Here, we report the design and construction of RNA nanostructures based on homooligomerizable one-stranded tiles for x-ray crystallographic determination. We solved three structures to near-atomic resolution: a 2D parallelogram, a 3D nanobracelet unexpectedly formed from an RNA designed for a nanocage, and, eventually, a bona fide 3D nanocage designed with the guidance of the two previous structures. Structural details of their constituent motifs, such as kissing loops, branched kissing loops, and T-junctions, that resemble natural RNA motifs and resisted x-ray determination are revealed, providing insights into those natural motifs. This work unveils the largely unexplored potential of crystallography in gaining high-resolution feedback for nanoarchitectural design and suggests a route to investigate RNA motif structures by configuring them into nanoarchitectures.

PubMed: 34550747
DOI: 10.1126/sciadv.abf4459

Experimental method

X-RAY DIFFRACTION (2.16 Å)