7JRO

Plant Mitochondrial complex IV from Vigna radiata

7JRO の概要

• エントリーDOI: 10.2210/pdb7jro/pdb
• 関連するPDBエントリー: 7JRG
• EMDBエントリー: 22445 22447
• 分子名称: COX1, COX7c, HEME-A, ... (19 entities in total)
• 機能のキーワード: mitochondria, respiration, bioenergetics, plants, oxidoreductase
• 由来する生物種: Vigna radiata (Mung bean); 詳細
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 216213.16

構造登録者

Maldonado, M.,Letts, J.A. (登録日: 2020-08-12, 公開日: 2021-01-20, 最終更新日: 2024-10-23)

主引用文献

Maldonado, M.,Guo, F.,Letts, J.A.
Atomic structures of respiratory complex III 2 , complex IV, and supercomplex III 2 -IV from vascular plants.
Elife, 10:-, 2021

PubMed Abstract: Mitochondrial complex III (CIII) and complex IV (CIV), which can associate into a higher-order supercomplex (SC III+IV), play key roles in respiration. However, structures of these plant complexes remain unknown. We present atomic models of CIII, CIV, and SC III+IV from determined by single-particle cryoEM. The structures reveal plant-specific differences in the MPP domain of CIII and define the subunit composition of CIV. Conformational heterogeneity analysis of CIII revealed long-range, coordinated movements across the complex, as well as the motion of CIII's iron-sulfur head domain. The CIV structure suggests that, in plants, proton translocation does not occur via the H channel. The supercomplex interface differs significantly from that in yeast and bacteria in its interacting subunits, angle of approach and limited interactions in the mitochondrial matrix. These structures challenge long-standing assumptions about the plant complexes and generate new mechanistic hypotheses.

PubMed: 33463523
DOI: 10.7554/eLife.62047

実験手法

ELECTRON MICROSCOPY (3.8 Å)