7JNH

Crystal structure of a double-ENE RNA stability element in complex with a 28-mer poly(A) RNA

7JNH の概要

• エントリーDOI: 10.2210/pdb7jnh/pdb
• 分子名称: 28-mer poly(A) RNA, Core double ENE RNA (Xtal construct) from Oryza sativa transposon,Core double ENE RNA (Xtal construct) from Oryza sativa transposon, STRONTIUM ION, ... (7 entities in total)
• 機能のキーワード: rna stability, element for nuclear expression (ene), poly(a) tail, major-groove triple helix, quintuple-base transition motif, wc-h a-minor interaction, poly(a) 3-end binding pocket, rna
• 由来する生物種: synthetic construct; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 39391.16

構造登録者

Torabi, S.F.,Vaidya, A.T.,Tycowski, K.T.,DeGregorio, S.J.,Wang, J.,Shu, M.D.,Steitz, T.A.,Steitz, J.A. (登録日: 2020-08-04, 公開日: 2021-01-20, 最終更新日: 2024-04-03)

主引用文献

Torabi, S.F.,Vaidya, A.T.,Tycowski, K.T.,DeGregorio, S.J.,Wang, J.,Shu, M.D.,Steitz, T.A.,Steitz, J.A.
RNA stabilization by a poly(A) tail 3'-end binding pocket and other modes of poly(A)-RNA interaction.
Science, 371:-, 2021

PubMed Abstract: Polyadenylate [poly(A)] tail addition to the 3' end of a wide range of RNAs is a highly conserved modification that plays a central role in cellular RNA function. Elements for nuclear expression (ENEs) are cis-acting RNA elements that stabilize poly(A) tails by sequestering them in RNA triplex structures. A crystal structure of a double ENE from a rice hAT transposon messenger RNA complexed with poly(A) at a resolution of 2.89 angstroms reveals multiple modes of interaction with poly(A), including major-groove triple helices, extended minor-groove interactions with RNA double helices, a quintuple-base motif that transitions poly(A) from minor-groove associations to major-groove triple helices, and a poly(A) 3'-end binding pocket. Our findings both expand the repertoire of motifs involved in long-range RNA interactions and provide insights into how polyadenylation can protect an RNA's extreme 3' end.

PubMed: 33414189
DOI: 10.1126/science.abe6523

実験手法

X-RAY DIFFRACTION (2.89 Å)