7JMH

Functional Pathways of Biomolecules Retrieved from Single-particle Snapshots - Frame 35 - State 4 (S4)

これはPDB形式変換不可エントリーです。

7JMH の概要

• エントリーDOI: 10.2210/pdb7jmh/pdb
• 関連するPDBエントリー: 6PV6 7JMF 7JMG 7JMI 7JMJ
• EMDBエントリー: 20486 22392 22393 22394 22395 22396
• 分子名称: Peptidyl-prolyl cis-trans isomerase FKBP1B, ryanodine receptor type 1, ZINC ION, ... (4 entities in total)
• 機能のキーワード: ion channel, ca2+ channel, excitation/contraction coupling, membrane protein
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 2056078.04

構造登録者

Dashti, A.,des Georges, A.,Frank, J.,Ourmazd, A. (登録日: 2020-07-31, 公開日: 2020-08-12, 最終更新日: 2024-03-06)

主引用文献

Dashti, A.,Mashayekhi, G.,Shekhar, M.,Ben Hail, D.,Salah, S.,Schwander, P.,des Georges, A.,Singharoy, A.,Frank, J.,Ourmazd, A.
Retrieving functional pathways of biomolecules from single-particle snapshots.
Nat Commun, 11:4734-4734, 2020

PubMed Abstract: A primary reason for the intense interest in structural biology is the fact that knowledge of structure can elucidate macromolecular functions in living organisms. Sustained effort has resulted in an impressive arsenal of tools for determining the static structures. But under physiological conditions, macromolecules undergo continuous conformational changes, a subset of which are functionally important. Techniques for capturing the continuous conformational changes underlying function are essential for further progress. Here, we present chemically-detailed conformational movies of biological function, extracted data-analytically from experimental single-particle cryo-electron microscopy (cryo-EM) snapshots of ryanodine receptor type 1 (RyR1), a calcium-activated calcium channel engaged in the binding of ligands. The functional motions differ substantially from those inferred from static structures in the nature of conformationally active structural domains, the sequence and extent of conformational motions, and the way allosteric signals are transduced within and between domains. Our approach highlights the importance of combining experiment, advanced data analysis, and molecular simulations.

PubMed: 32948759
DOI: 10.1038/s41467-020-18403-x

実験手法

ELECTRON MICROSCOPY (4.5 Å)