7JJU

Crystal structure of en exoribonuclease-resistant RNA (xrRNA) from Potato leafroll virus (PLRV)

7JJU の概要

• エントリーDOI: 10.2210/pdb7jju/pdb
• 分子名称: Potato leafroll virus xrRNA, Guanidinium, CACODYLATE ION, ... (4 entities in total)
• 機能のキーワード: rna structure viral rna exonuclease resistance rna pseudoknot polerovirus plant virus, rna
• 由来する生物種: Potato leafroll virus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 37590.10

構造登録者

Steckelberg, A.-L.,Vicens, Q.,Auffinger, P.,Costantino, D.C.,Nix, J.C.,Kieft, J.S. (登録日: 2020-07-27, 公開日: 2020-09-09, 最終更新日: 2024-03-06)

主引用文献

Steckelberg, A.L.,Vicens, Q.,Costantino, D.A.,Nix, J.C.,Kieft, J.S.
The crystal structure of a Polerovirus exoribonuclease-resistant RNA shows how diverse sequences are integrated into a conserved fold.
Rna, 26:1767-1776, 2020

PubMed Abstract: Exoribonuclease-resistant RNAs (xrRNAs) are discrete elements that block the progression of 5' to 3' exoribonucleases using specifically folded RNA structures. A recently discovered class of xrRNA is widespread in several genera of plant-infecting viruses, within both noncoding and protein-coding subgenomic RNAs. The structure of one such xrRNA from a dianthovirus revealed three-dimensional details of the resistant fold but did not answer all questions regarding the conservation and diversity of this xrRNA class. Here, we present the crystal structure of a representative polerovirus xrRNA that contains sequence elements that diverge from the previously solved structure. This new structure rationalizes previously unexplained sequence conservation patterns and shows interactions not present in the first structure. Together, the structures of these xrRNAs from dianthovirus and polerovirus genera support the idea that these plant virus xrRNAs fold through a defined pathway that includes a programmed intermediate conformation. This work deepens our knowledge of the structure-function relationship of xrRNAs and shows how evolution can craft similar RNA folds from divergent sequences.

PubMed: 32848042
DOI: 10.1261/rna.076224.120

実験手法

X-RAY DIFFRACTION (2.604 Å)