7FJ2

Structure of FOXM1 homodimer bound to a palindromic DNA site

7FJ2 の概要

• エントリーDOI: 10.2210/pdb7fj2/pdb
• 分子名称: Forkhead box protein M1, DNA (5'-D(*AP*CP*CP*GP*TP*AP*AP*AP*CP*AP*TP*GP*TP*TP*TP*AP*CP*GP*GP*T)-3') (2 entities in total)
• 機能のキーワード: forkhead transcription factors, foxm1, dimer, dna binding, transcription
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 79253.91

構造登録者

Dai, S.Y.,Li, J.,Zhang, H.J. (登録日: 2021-08-02, 公開日: 2022-01-26, 最終更新日: 2023-11-29)

主引用文献

Zhang, H.,Dai, S.,Liang, X.,Li, J.,Chen, Y.
Mechanistic Insights into the Preference for Tandem Binding Sites in DNA Recognition by FOXM1.
J.Mol.Biol., 434:167426-167426, 2021

PubMed Abstract: FOXM1 is an essential proliferation-associated transcription factor that controls the activation of a number of cell cycle regulatory genes. Unlike other forkhead box (FOX) transcription factors, FOXM1 has been shown to prefer binding tandem regulatory DNA sites. However, the underlying reason for such preference is not clear. Here, we showed that the tandem DNA motif, named DIV2, is widely distributed in the promoter region of FOXM1 target genes. The binding of FOXM1 on the DIV2 site differs dramatically from other sites, which is in a highly cooperative fashion, with a much enhanced thermal stability and can be clearly detected by EMSA. The crystal structure of FOXM1 in complex with the DIV2 DNA reveals that the cooperative binding is likely to be driven by intermolecular protein-protein interactions (PPIs). Further half-site spacer insertion assays showed that FOXM1 can bind another site, DIV0, in a similar manner to the DIV2 site. Given the high occurrence of the DIV2 and DIV0 sites in FOXM1 target genes, our results suggest that FOXM1 prefers tandem DNA sites to enable cooperative DNA recognition, and such binding characteristics may further confer its specificity during transcriptional regulation.

PubMed: 34973238
DOI: 10.1016/j.jmb.2021.167426

実験手法

X-RAY DIFFRACTION (3.098 Å)