7FIW

Crystal structure of the complex formed by Wolbachia cytoplasmic incompatibility factors CidAwMel(ST) and CidBND1-ND2 from wPip(Pel)

Summary for 7FIW

• Entry DOI: 10.2210/pdb7fiw/pdb
• Descriptor: ULP_PROTEASE domain-containing protein, bacteria factor 4,CidA I(Zeta/1) protein (3 entities in total)
• Functional Keywords: wolbachia, cytoplasmic incompatibility, antitoxin, protein binding
• Biological source: Wolbachia endosymbiont of Culex quinquefasciatus Pel; More
• Total number of polymer chains: 2
• Total formula weight: 144838.20

Authors

Xiao, Y.J.,Wang, W.,Chen, X.,Ji, X.Y.,Yang, H.T. (deposition date: 2021-08-01, release date: 2022-04-06, Last modification date: 2023-11-29)

Primary citation

Wang, H.,Xiao, Y.,Chen, X.,Zhang, M.,Sun, G.,Wang, F.,Wang, L.,Zhang, H.,Zhang, X.,Yang, X.,Li, W.,Wei, Y.,Yao, D.,Zhang, B.,Li, J.,Cui, W.,Wang, F.,Chen, C.,Shen, W.,Su, D.,Bai, F.,Huang, J.,Ye, S.,Zhang, L.,Ji, X.,Wang, W.,Wang, Z.,Hochstrasser, M.,Yang, H.
Crystal Structures of Wolbachia CidA and CidB Reveal Determinants of Bacteria-induced Cytoplasmic Incompatibility and Rescue.
Nat Commun, 13:1608-1608, 2022

PubMed Abstract: Cytoplasmic incompatibility (CI) results when Wolbachia bacteria-infected male insects mate with uninfected females, leading to embryonic lethality. "Rescue" of viability occurs if the female harbors the same Wolbachia strain. CI is caused by linked pairs of Wolbachia genes called CI factors (CifA and CifB). The co-evolution of CifA-CifB pairs may account in part for the incompatibility patterns documented in insects infected with different Wolbachia strains, but the molecular mechanisms remain elusive. Here, we use X-ray crystallography and AlphaFold to analyze the CI factors from Wolbachia strain wMel called CidA and CidB. Substituting CidA interface residues with those from CidA (from strain wPip) enables the mutant protein to bind CidB and rescue CidB-induced yeast growth defects, supporting the importance of CifA-CifB interaction in CI rescue. Sequence divergence in CidA and CidB proteins affects their pairwise interactions, which may help explain the complex incompatibility patterns of mosquitoes infected with different wPip strains.

PubMed: 35338130
DOI: 10.1038/s41467-022-29273-w

Experimental method

X-RAY DIFFRACTION (2.16 Å)