7FGI

Cryo-EM Structure of Chikungunya Virus Nonstructural Protein 1 with m7Gppp-AU

7FGI の概要

• エントリーDOI: 10.2210/pdb7fgi/pdb
• EMDBエントリー: 31582
• 分子名称: mRNA-capping enzyme nsP1, m7Gppp-AU, ZINC ION, ... (5 entities in total)
• 機能のキーワード: nonstructural protein, chikungunya virus, rna cap, replication, viral protein, viral protein-rna complex, viral protein/rna
• 由来する生物種: Chikungunya virus strain S27-African prototype; 詳細
• タンパク質・核酸の鎖数: 24
• 化学式量合計: 766073.30

構造登録者

Zhang, K.,Law, M.C.Y.,Nguyen, T.M.,Tan, Y.B.,Wirawan, M.,Law, Y.S.,Luo, D.H. (登録日: 2021-07-27, 公開日: 2022-07-27, 最終更新日: 2024-06-05)

主引用文献

Zhang, K.,Law, M.C.Y.,Nguyen, T.M.,Tan, Y.B.,Wirawan, M.,Law, Y.S.,Jeong, L.S.,Luo, D.
Molecular basis of specific viral RNA recognition and 5'-end capping by the Chikungunya virus nsP1.
Cell Rep, 40:111133-111133, 2022

PubMed Abstract: Many viruses encode RNA-modifying enzymes to edit the 5' end of viral RNA to mimic the cellular mRNA for effective protein translation, genome replication, and evasion of the host defense mechanisms. Alphavirus nsP1 synthesizes the 5' end Cap-0 structure of viral RNAs. However, the molecular basis of the capping process remains unclear. We determine high-resolution cryoelectron microscopy (cryo-EM) structures of Chikungunya virus nsP1 in complex with m7GTP/SAH, covalently attached m7GMP, and Cap-0 viral RNA. These structures reveal details of viral-RNA-capping reactions and uncover a sequence-specific virus RNA-recognition pattern that, in turn, regulates viral-RNA-capping efficiency to ensure optimal genome replication and subgenomic RNA transcription. This sequence-specific enzyme-RNA pairing is conserved across all alphaviruses.

PubMed: 35905713
DOI: 10.1016/j.celrep.2022.111133

実験手法

ELECTRON MICROSCOPY (2.51 Å)