7FFU
Osmium-bound human serum transferrin
Summary for 7FFU
| Entry DOI | 10.2210/pdb7ffu/pdb |
| Related | 5WTD |
| Descriptor | Serotransferrin, FE (III) ION, MALONATE ION, ... (5 entities in total) |
| Functional Keywords | osmium, tranferrin, iron transport, metal transport |
| Biological source | Homo sapiens (Human) |
| Total number of polymer chains | 1 |
| Total formula weight | 75632.60 |
| Authors | |
| Primary citation | Wang, M.,Wang, H.,Xu, X.,Lai, T.P.,Zhou, Y.,Hao, Q.,Li, H.,Sun, H. Binding of ruthenium and osmium at non‐iron sites of transferrin accounts for their iron-independent cellular uptake. J.Inorg.Biochem., 234:111885-111885, 2022 Cited by PubMed Abstract: Being identified with less toxic and generally showing selective effects for solid tumor metastases, ruthenium and osmium compounds are promising drug candidates for clinical uses. Human serum proteins, such as albumin and transferrin, play vital roles in the transportation and accumulation of ruthenium and osmium agents into target tissues. However, the molecular mechanism of how transferrin transport ruthenium and their osmium analogues at atomic level remains obscure. In this study, we uncovered that the cellular uptake of Os or Ru are not competed by Fe. To unveil the molecular mechanism behind the phenomena, we report the first crystal structures of human serum transferrin (hTF) in complex with ruthenium and osmium compounds bound to the non-conserved residues on the surface of hTF without altering its overall conformation. As for Ru and Os, these binding sites by descending affinity are: His14/His289, His349-350 ~ His578/Arg581. Ruthenium drugs and their osmium analogues preferentially bind to His14/His289 with bipyridine or imidazole ligands leaving. These binding sites on hTF surface are also available in human lactoferrin and some transferrin family member of other species. The presence of these binding sites makes the cellular uptake of Ru and Os less affected by Fe, compare to Zr or Hf. Collectively, these findings are critical for our understanding of the role of serum transferrin in cellular delivery of ruthenium and osmium anticancer agents. PubMed: 35690040DOI: 10.1016/j.jinorgbio.2022.111885 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.601 Å) |
Structure validation
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