7FF8

Pseudomonas aeruginosa Virulence Factor Regulator with cAMP ligand and Cl(triethylphosphine)gold(I)

Summary for 7FF8

• Entry DOI: 10.2210/pdb7ff8/pdb
• Descriptor: cAMP-activated global transcriptional regulator Vfr, ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE, triethylphosphanuidylgold(1+), ... (6 entities in total)
• Functional Keywords: camp, auranofin, virulence, cyclic amp receptor protein, dna binding protein
• Biological source: Pseudomonas aeruginosa PAO1
• Total number of polymer chains: 2
• Total formula weight: 50427.67

Authors

Chew, B.L.A.,Luo, D. (deposition date: 2021-07-22, release date: 2022-07-27, Last modification date: 2025-03-05)

Primary citation

Zhang, Y.,Chew, B.L.A.,Wang, J.,Yuan, M.,Yam, J.K.H.,Luo, D.,Yang, L.
Structural basis for the inhibitory mechanism of auranofin and gold(I) analogues against Pseudomonas aeruginosa global virulence factor regulator Vfr.
Comput Struct Biotechnol J, 21:2137-2146, 2023

PubMed Abstract: is a leading cause of hospital-acquired infections. Treatment of infections is difficult given its multiple virulence mechanisms, intrinsic antibiotic resistance mechanisms, and biofilm-forming ability. Auranofin, an approved oral gold compound for rheumatoid arthritis treatment, was recently reported to inhibit the growth of multiple bacterial species. Here, we identify 's global virulence factor regulator Vfr as one target of auranofin. We report the mechanistic insights into the inhibitory mechanism of auranofin and gold(I) analogues to Vfr through structural, biophysical, and phenotypic inhibition studies. This work suggests that auranofin and gold(I) analogues have potential to be developed as anti-virulence drugs against

PubMed: 37007650
DOI: 10.1016/j.csbj.2023.03.013

Experimental method

X-RAY DIFFRACTION (2.8 Å)