7FDF

The E145S mutant of the regulatory domain of YeiE, a sulfite sensing LysR-type transcriptional regulator from Cronobacter sakazakii (sulfate-bound form)

7FDF の概要

• エントリーDOI: 10.2210/pdb7fdf/pdb
• 関連するPDBエントリー: 7ERP 7ERQ
• 分子名称: LysR family transcriptional regulator, SULFATE ION (3 entities in total)
• 機能のキーワード: lysr-type transcriptional regulator, lttr, cronobacter sakazakii, sulfite, dna binding protein
• 由来する生物種: Cronobacter sakazakii (Enterobacter sakazakii)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 23318.00

構造登録者

Hong, S.,Ha, N.-C. (登録日: 2021-07-16, 公開日: 2022-03-23, 最終更新日: 2023-11-29)

主引用文献

Hong, S.,Kim, J.,Cho, E.,Na, S.,Yoo, Y.J.,Cho, Y.H.,Ryu, S.,Ha, N.C.
Crystal structures of YeiE from Cronobacter sakazakii and the role of sulfite tolerance in gram-negative bacteria.
Proc.Natl.Acad.Sci.USA, 119:e2118002119-e2118002119, 2022

PubMed Abstract: SignificanceYeiE has been identified as a master virulence factor of . In this study, we determined the crystal structures of the regulatory domain of YeiE in complex with its physiological ligand sulfite ion (SO). The structure provides the basis for the molecular mechanisms for sulfite sensing and the ligand-dependent conformational changes of the regulatory domain. The genes under the control of YeiE in response to sulfite were investigated to reveal the functional roles of YeiE in the sulfite tolerance of the bacteria. We propose the molecular mechanism underlying the ability of gram-negative pathogens to defend against the innate immune response involving sulfite, thus providing a strategy to control the pathogenesis of bacteria.

PubMed: 35271389
DOI: 10.1073/pnas.2118002119

実験手法

X-RAY DIFFRACTION (2.05 Å)