7FD9

Thermostabilised full length human mGluR5-5M with orthosteric antagonist, LY341495

7FD9 の概要

• エントリーDOI: 10.2210/pdb7fd9/pdb
• 関連するPDBエントリー: 7P2L
• EMDBエントリー: 31536 31537
• 分子名称: Metabotropic glutamate receptor 5, 2-acetamido-2-deoxy-beta-D-glucopyranose, 2-[(1S,2S)-2-carboxycyclopropyl]-3-(9H-xanthen-9-yl)-D-alanine (3 entities in total)
• 機能のキーワード: g-protein coupled receptors, signal transduction, metabotropic glutamate receptor, inactive state, membrane protein
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 195057.11

構造登録者

Vinothkumar, K.R.,Cannone, G.,Lebon, G. (登録日: 2021-07-16, 公開日: 2021-09-08, 最終更新日: 2024-11-13)

主引用文献

Nasrallah, C.,Cannone, G.,Briot, J.,Rottier, K.,Berizzi, A.E.,Huang, C.Y.,Quast, R.B.,Hoh, F.,Baneres, J.L.,Malhaire, F.,Berto, L.,Dumazer, A.,Font-Ingles, J.,Gomez-Santacana, X.,Catena, J.,Kniazeff, J.,Goudet, C.,Llebaria, A.,Pin, J.P.,Vinothkumar, K.R.,Lebon, G.
Agonists and allosteric modulators promote signaling from different metabotropic glutamate receptor 5 conformations.
Cell Rep, 36:109648-109648, 2021

PubMed Abstract: Metabotropic glutamate receptors (mGluRs) are dimeric G-protein-coupled receptors activated by the main excitatory neurotransmitter, L-glutamate. mGluR activation by agonists binding in the venus flytrap domain is regulated by positive (PAM) or negative (NAM) allosteric modulators binding to the 7-transmembrane domain (7TM). We report the cryo-electron microscopy structures of fully inactive and intermediate-active conformations of mGlu receptor bound to an antagonist and a NAM or an agonist and a PAM, respectively, as well as the crystal structure of the 7TM bound to a photoswitchable NAM. The agonist induces a large movement between the subunits, bringing the 7TMs together and stabilizing a 7TM conformation structurally similar to the inactive state. Using functional approaches, we demonstrate that the PAM stabilizes a 7TM active conformation independent of the conformational changes induced by agonists, representing an alternative mode of mGlu activation. These findings provide a structural basis for different mGluR activation modes.

PubMed: 34469715
DOI: 10.1016/j.celrep.2021.109648

実験手法

ELECTRON MICROSCOPY (4 Å)