7FCF

Crystal structure of T6SS Hcp protein

これはPDB形式変換不可エントリーです。

7FCF の概要

• エントリーDOI: 10.2210/pdb7fcf/pdb
• 分子名称: Fimbrial protein (2 entities in total)
• 機能のキーワード: hexamer, structural protein
• 由来する生物種: Chromobacterium haemolyticum; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 105701.47

構造登録者

Jobichen, C.,Sivaraman, J. (登録日: 2021-07-14, 公開日: 2022-07-20, 最終更新日: 2023-11-29)

主引用文献

Zwe, Y.H.,Yadav, M.,Ten, M.M.Z.,Srinivasan, M.,Jobichen, C.,Sivaraman, J.,Li, D.
Bacterial antagonism of Chromobacterium haemolyticum and characterization of its putative type VI secretion system.
Res.Microbiol., 173:103918-103918, 2022

PubMed Abstract: This study reports the isolation of a new Chromobacterium haemolyticum strain named WI5 from a hydroponic farming facility. WI5 exhibited remarkable bacterial antagonistic properties, eliminating Salmonella, Escherichia coli, Listeria monocytogenes and Staphylococcus aureus (initial inoculum load ∼10 CFU/ml) in dual-species co-culture biofilms. Antagonism was strictly contact-dependent and highly influenced by nutrient availability. Next, we identified a complete suite of putative Type VI secretion system (T6SS) genes in the WI5 genome, annotated the gene locus architecture, and determined the crystal structure of hallmark T6SS tube protein Hcp1, which revealed a hexameric ring structure with an outer and inner diameter of 77 and 45 Å, respectively. Structural comparison with homologs showed differences in the key loops connecting the β-strands in which the conserved residues are located, suggesting a role of these residues in the protein function. The T6SS is well-known to facilitate interbacterial competition, and the putative T6SS characterized herein might be responsible for the remarkable antagonism by C. haemolyticum WI5. Collectively, these findings shed light on the nature of bacterial antagonism and a putative key virulence determinant of C. haemolyticum, which might aid in further understanding its potential ecological role in natural habitats.

PubMed: 34906677
DOI: 10.1016/j.resmic.2021.103918

実験手法

X-RAY DIFFRACTION (3.3 Å)