7F63

Cryo-EM structure of SARS-CoV-2 spike in complex with a neutralizing antibody chAb-45 (Focused refinement of S-RBD and chAb-45 region)

7F63 の概要

• エントリーDOI: 10.2210/pdb7f63/pdb
• 関連するPDBエントリー: 7EJ5
• EMDBエントリー: 30670 31471
• 分子名称: Spike glycoprotein, RBD-chAb45, Heavy chain, RBD-chAb45, Light chain (3 entities in total)
• 機能のキーワード: sars-cov-2, spike protein, neutralizing antibody, viral protein
• 由来する生物種: Severe acute respiratory syndrome coronavirus 2 (2019-nCoV, SARS-CoV-2); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 215170.65

構造登録者

Yang, T.J.,Yu, P.Y.,Wu, H.C.,Hsu, S.T.D. (登録日: 2021-06-24, 公開日: 2021-08-04, 最終更新日: 2024-10-23)

主引用文献

Su, S.C.,Yang, T.J.,Yu, P.Y.,Liang, K.H.,Chen, W.Y.,Yang, C.W.,Lin, H.T.,Wang, M.J.,Lu, R.M.,Tso, H.C.,Chung, M.J.,Hsieh, T.Y.,Chang, Y.L.,Lin, S.C.,Hsu, F.Y.,Ke, F.Y.,Wu, Y.H.,Hwang, Y.C.,Liu, I.J.,Liang, J.J.,Liao, C.C.,Ko, H.Y.,Sun, C.P.,Wu, P.Y.,Jan, J.T.,Chang, Y.C.,Lin, Y.L.,Tao, M.H.,Hsu, S.D.,Wu, H.C.
Structure-guided antibody cocktail for prevention and treatment of COVID-19.
Plos Pathog., 17:e1009704-e1009704, 2021

PubMed Abstract: Development of effective therapeutics for mitigating the COVID-19 pandemic is a pressing global need. Neutralizing antibodies are known to be effective antivirals, as they can be rapidly deployed to prevent disease progression and can accelerate patient recovery without the need for fully developed host immunity. Here, we report the generation and characterization of a series of chimeric antibodies against the receptor-binding domain (RBD) of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein. Some of these antibodies exhibit exceptionally potent neutralization activities in vitro and in vivo, and the most potent of our antibodies target three distinct non-overlapping epitopes within the RBD. Cryo-electron microscopy analyses of two highly potent antibodies in complex with the SARS-CoV-2 spike protein suggested they may be particularly useful when combined in a cocktail therapy. The efficacy of this antibody cocktail was confirmed in SARS-CoV-2-infected mouse and hamster models as prophylactic and post-infection treatments. With the emergence of more contagious variants of SARS-CoV-2, cocktail antibody therapies hold great promise to control disease and prevent drug resistance.

PubMed: 34673836
DOI: 10.1371/journal.ppat.1009704

実験手法

ELECTRON MICROSCOPY (3.9 Å)