7F5K

Crystal structure of TCR4-1 ectodomain

Summary for 7F5K

• Entry DOI: 10.2210/pdb7f5k/pdb
• Descriptor: T cell receptor alpha chain, T cell receptor beta chain (2 entities in total)
• Functional Keywords: t-cell receptor, ectodomain, immune system
• Biological source: Mus musculus; More
• Total number of polymer chains: 4
• Total formula weight: 96972.04

Authors

Nagae, M.,Yamasaki, S. (deposition date: 2021-06-22, release date: 2022-06-22, Last modification date: 2024-11-13)

Primary citation

Shibata, K.,Motozono, C.,Nagae, M.,Shimizu, T.,Ishikawa, E.,Motooka, D.,Okuzaki, D.,Izumi, Y.,Takahashi, M.,Fujimori, N.,Wing, J.B.,Hayano, T.,Asai, Y.,Bamba, T.,Ogawa, Y.,Furutani-Seiki, M.,Shirai, M.,Yamasaki, S.
Symbiotic bacteria-dependent expansion of MR1-reactive T cells causes autoimmunity in the absence of Bcl11b.
Nat Commun, 13:6948-6948, 2022

PubMed Abstract: MHC class I-related protein 1 (MR1) is a metabolite-presenting molecule that restricts MR1-reactive T cells including mucosal-associated invariant T (MAIT) cells. In contrast to MAIT cells, the function of other MR1-restricted T cell subsets is largely unknown. Here, we report that mice in which a T cell-specific transcription factor, B-cell lymphoma/leukemia 11B (Bcl11b), was ablated in immature thymocytes (Bcl11b mice) develop chronic inflammation. Bcl11b mice lack conventional T cells and MAIT cells, whereas CD4IL-18R αβ T cells expressing skewed Traj33 (Jα33) T cell receptors (TCR) accumulate in the periphery, which are necessary and sufficient for the pathogenesis. The disorders observed in Bcl11b mice are ameliorated by MR1-deficiency, transfer of conventional T cells, or germ-free conditions. We further show the crystal structure of the TCR expressed by Traj33 T cells expanded in Bcl11b mice. Overall, we establish that MR1-reactive T cells have pathogenic potential.

PubMed: 36376329
DOI: 10.1038/s41467-022-34802-8

Experimental method

X-RAY DIFFRACTION (3.00002976572 Å)