7F3B

cocrystallization of Escherichia coli dihydrofolate reductase (DHFR) and its pyrrolo[3,2-f]quinazoline inhibitor.

7F3B の概要

• エントリーDOI: 10.2210/pdb7f3b/pdb
• 分子名称: Dihydrofolate reductase, 7-[(2-fluorophenyl)methyl]pyrrolo[3,2-f]quinazoline-1,3-diamine, GLYCEROL (3 entities in total)
• 機能のキーワード: folate pathway, dihydrofolate reductase, catalyze the reduction of dihydrofolate to tetrahydrofolate, antibiotic
• 由来する生物種: Escherichia coli (strain K12)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 19339.74

構造登録者

Wang, H.,You, X.F.,Yang, X.Y.,Li, Y.,Hong, W. (登録日: 2021-06-16, 公開日: 2022-04-27, 最終更新日: 2023-11-29)

主引用文献

Li, Y.,Ouyang, Y.,Wu, H.,Wang, P.,Huang, Y.,Li, X.,Chen, H.,Sun, Y.,Hu, X.,Wang, X.,Li, G.,Lu, Y.,Li, C.,Lu, X.,Pang, J.,Nie, T.,Sang, X.,Dong, L.,Dong, W.,Jiang, J.,Paterson, I.C.,Yang, X.,Hong, W.,Wang, H.,You, X.
The discovery of 1, 3-diamino-7H-pyrrol[3, 2-f]quinazoline compounds as potent antimicrobial antifolates.
Eur.J.Med.Chem., 228:113979-113979, 2022

PubMed Abstract: The shortage of new antibiotics makes infections caused by gram-negative (G) bacteria a significant clinical problem. The key enzymes involved in folate biosynthesis represent important targets for drug discovery, and new antifolates with novel mechanisms are urgently needed. By targeting to dihydrofolate reductase (DHFR), a series of 1,3-diamino-7H-pyrrol[3,2-f]quinazoline (PQZ) compounds were designed, and exhibited potent antibacterial activities in vitro, especially against multi-drug resistant G strains. Multiple experiments indicated that PQZ compounds contain a different molecular mechanism against the typical DHFR inhibitor, trimethoprim (TMP), and the thymidylate synthase (TS) was identified as another potential but a relatively weak target. A significant synergism between the representative compound, OYYF-175, and sulfamethoxazole (SMZ) was observed with a strong cumulative and significantly bactericidal effect at extremely low concentrations (2 μg/mL for SMZ and 0.03 pg/mL for OYYF-175), which could be resulted from the simultaneous inhibition of dihydropteroate synthase (DHPS), DHFR and TS. PQZ compounds exhibited therapeutic effects in a mouse model of intraperitoneal infections caused by Escherichia coli (E. coli). The co-crystal structure of OYYF-175-DHFR was solved and the detailed interactions were provided. The inhibitors reported represent innovative chemical structures with novel molecular mechanism of action, which will benefit the generation of new, efficacious bactericidal compounds.

PubMed: 34802838
DOI: 10.1016/j.ejmech.2021.113979

実験手法

X-RAY DIFFRACTION (2.81 Å)