7F1M

Marburg virus nucleoprotein-RNA complex

7F1M の概要

• エントリーDOI: 10.2210/pdb7f1m/pdb
• EMDBエントリー: 31420
• 分子名称: Nucleoprotein, RNA (5'-R(P*UP*UP*UP*UP*UP*U)-3') (2 entities in total)
• 機能のキーワード: nucleoprotein, viral protein
• 由来する生物種: Lake Victoria marburgvirus (strain Angola/2005) (MARV); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 91914.74

構造登録者

Fujita, F.Y.,Sugita, Y.,Takamatsu, Y.,Houri, K.,Muramoto, Y.,Nakano, M.,Tsunoda, Y.,Igarashi, M.,Becker, S.,Noda, T. (登録日: 2021-06-09, 公開日: 2022-03-09, 最終更新日: 2024-06-12)

主引用文献

Fujita-Fujiharu, Y.,Sugita, Y.,Takamatsu, Y.,Houri, K.,Igarashi, M.,Muramoto, Y.,Nakano, M.,Tsunoda, Y.,Taniguchi, I.,Becker, S.,Noda, T.
Structural insight into Marburg virus nucleoprotein-RNA complex formation.
Nat Commun, 13:1191-1191, 2022

PubMed Abstract: The nucleoprotein (NP) of Marburg virus (MARV), a close relative of Ebola virus (EBOV), encapsidates the single-stranded, negative-sense viral genomic RNA (vRNA) to form the helical NP-RNA complex. The NP-RNA complex constitutes the core structure for the assembly of the nucleocapsid that is responsible for viral RNA synthesis. Although appropriate interactions among NPs and RNA are required for the formation of nucleocapsid, the structural basis of the helical assembly remains largely elusive. Here, we show the structure of the MARV NP-RNA complex determined using cryo-electron microscopy at a resolution of 3.1 Å. The structures of the asymmetric unit, a complex of an NP and six RNA nucleotides, was very similar to that of EBOV, suggesting that both viruses share common mechanisms for the nucleocapsid formation. Structure-based mutational analysis of both MARV and EBOV NPs identified key residues for helical assembly and subsequent viral RNA synthesis. Importantly, most of the residues identified were conserved in both viruses. These findings provide a structural basis for understanding the nucleocapsid formation and contribute to the development of novel antivirals against MARV and EBOV.

PubMed: 35246537
DOI: 10.1038/s41467-022-28802-x

実験手法

ELECTRON MICROSCOPY (3.1 Å)