7F1E

Structure of METTL6 bound with SAM

7F1E の概要

• エントリーDOI: 10.2210/pdb7f1e/pdb
• 分子名称: tRNA N(3)-methylcytidine methyltransferase METTL6, S-ADENOSYLMETHIONINE (3 entities in total)
• 機能のキーワード: rna methyltransferase, rna, transferase
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 67779.37

構造登録者

Li, S.,Liao, S.,Xu, C. (登録日: 2021-06-09, 公開日: 2022-01-12, 最終更新日: 2023-11-29)

主引用文献

Li, S.,Zhou, H.,Liao, S.,Wang, X.,Zhu, Z.,Zhang, J.,Xu, C.
Structural basis for METTL6-mediated m3C RNA methylation.
Biochem.Biophys.Res.Commun., 589:159-164, 2021

PubMed Abstract: RNA modifications play important roles in mediating the biological functions of RNAs. 3-methylcytidine (m3C), albeit less abundant, is found to exist extensively in tRNAs, rRNAs and mRNAs. Human METTL6 is a mC methyltransferase for tRNAs, including tRNA. We solved the structure of human METTL6 in the presence of S-adenosyl-L-methionine and found by enzyme assay that recombinant human METTL6 is active towards tRNA. Structural analysis indicated the detailed interactions between S-adenosyl-L-methionine and METTL6, and suggested potential tRNA binding region on the surface of METTL6. The structural research, complemented by biochemistry enzyme assay, will definitely shed light on the design of potent inhibitors for METTL6 in near future.

PubMed: 34922197
DOI: 10.1016/j.bbrc.2021.12.013

実験手法

X-RAY DIFFRACTION (2.589 Å)