7EYW

Fe(II)/(alpha)ketoglutarate-dependent dioxygenase SptF with terretonin C

7EYW の概要

• エントリーDOI: 10.2210/pdb7eyw/pdb
• 分子名称: 2-oxoglutarate/Fe(II)-dependent dioxygenase SptF, N-OXALYLGLYCINE, FE (II) ION, ... (5 entities in total)
• 機能のキーワード: alpha-ketoglutarate dependent dioxygenase, meroterpenoid, oxidoreductase
• 由来する生物種: Aspergillus sp.
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 136550.95

構造登録者

Tao, H.,Mori, T.,Abe, I. (登録日: 2021-06-01, 公開日: 2022-04-20, 最終更新日: 2023-11-29)

主引用文献

Tao, H.,Mori, T.,Chen, H.,Lyu, S.,Nonoyama, A.,Lee, S.,Abe, I.
Molecular insights into the unusually promiscuous and catalytically versatile Fe(II)/ alpha-ketoglutarate-dependent oxygenase SptF.
Nat Commun, 13:95-95, 2022

PubMed Abstract: Non-heme iron and α-ketoglutarate-dependent (Fe/αKG) oxygenases catalyze various oxidative biotransformations. Due to their catalytic flexibility and high efficiency, Fe/αKG oxygenases have attracted keen attention for their application as biocatalysts. Here, we report the biochemical and structural characterizations of the unusually promiscuous and catalytically versatile Fe/αKG oxygenase SptF, involved in the biosynthesis of fungal meroterpenoid emervaridones. The in vitro analysis revealed that SptF catalyzes several continuous oxidation reactions, including hydroxylation, desaturation, epoxidation, and skeletal rearrangement. SptF exhibits extremely broad substrate specificity toward various meroterpenoids, and efficiently produced unique cyclopropane-ring-fused 5/3/5/5/6/6 and 5/3/6/6/6 scaffolds from terretonins. Moreover, SptF also hydroxylates steroids, including androsterone, testosterone, and progesterone, with different regiospecificities. Crystallographic and structure-based mutagenesis studies of SptF revealed the molecular basis of the enzyme reactions, and suggested that the malleability of the loop region contributes to the remarkable substrate promiscuity. SptF exhibits great potential as a promising biocatalyst for oxidation reactions.

PubMed: 35013177
DOI: 10.1038/s41467-021-27636-3

実験手法

X-RAY DIFFRACTION (2.1 Å)