7EYO

Crystal structure of leech hyaluronidase

7EYO の概要

• エントリーDOI: 10.2210/pdb7eyo/pdb
• 分子名称: Hyaluronoglucuronidase, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: hyaluronidae, gh79, hydrolase
• 由来する生物種: Hirudo nipponia (Korean blood-sucking leech)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 57155.11

構造登録者

Huang, H.,Hou, X.D.,Rao, Y.J.,Kang, Z. (登録日: 2021-05-31, 公開日: 2022-05-25, 最終更新日: 2024-10-16)

主引用文献

Huang, H.,Hou, X.,Xu, R.,Deng, Z.,Wang, Y.,Du, G.,Rao, Y.,Chen, J.,Kang, Z.
Structure and cleavage pattern of a hyaluronate 3-glycanohydrolase in the glycoside hydrolase 79 family.
Carbohydr Polym, 277:118838-118838, 2022

PubMed Abstract: Hyaluronidases have attracted a great deal of interest in the field of medicine due to their fundamental roles in the breakdown of hyaluronan. However, little is known about the catalytic mechanism of the hyaluronate 3-glycanohydrolases. Here, we report the crystal structure and cleavage pattern of a leech hyaluronidase (LHyal), which hydrolyzes the β-1,3-glycosidic bonds of hyaluronan. LHyal exhibits the typical structural features of glycoside hydrolase 79 family but contains a variable 'exo-pocket' loop where basic residues R102 and K103 are the structural determinants of hyaluronan binding. Through analysis of the hydrolysis of even- and odd-numbered hyaluronan oligosaccharides, we demonstrate that hexasaccharide is the shortest natural substrate, which can be cleaved from both the reducing and non-reducing ends to release disaccharides, and pentasaccharides are the smallest fragments for recognition and hydrolysis. These observations provide new insights into the degradation of hyaluronan and the evolutionary relationships of the GH79 family enzymes.

PubMed: 34893255
DOI: 10.1016/j.carbpol.2021.118838

実験手法

X-RAY DIFFRACTION (1.85 Å)