7EXM

The N-terminal crystal structure of SARS-CoV-2 NSP2

7EXM の概要

• エントリーDOI: 10.2210/pdb7exm/pdb
• 分子名称: Non-structural protein 2, ZINC ION, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: zinc finger, nucleic acid binding protein, viral protein
• 由来する生物種: Severe acute respiratory syndrome coronavirus 2 (2019-nCoV, SARS-CoV-2)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 124969.95

構造登録者

Ma, J.,Chen, Z. (登録日: 2021-05-27, 公開日: 2021-06-16, 最終更新日: 2024-05-29)

主引用文献

Ma, J.,Chen, Y.,Wu, W.,Chen, Z.
Structure and Function of N-Terminal Zinc Finger Domain of SARS-CoV-2 NSP2.
Virol Sin, 36:1104-1112, 2021

PubMed Abstract: SARS-CoV-2 has become a global pandemic threatening human health and safety. It is urgent to find effective therapeutic agents and targets with the continuous emergence of novel mutant strains. The knowledge of the molecular basis and pathogenesis of SARS-CoV-2 in host cells requires to be understood comprehensively. The unknown structure and function of nsp2 have hindered our understanding of its role in SARS-CoV-2 infection. Here, we report the crystal structure of the N-terminal of SARS-CoV-2 nsp2 to a high resolution of 1.96 Å. This novel structure contains three zinc fingers, belonging to the C2H2, C4, and C2HC types, respectively. Structure analysis suggests that nsp2 may be involved in binding nucleic acids and regulating intracellular signaling pathways. The binding to single or double-stranded nucleic acids was mainly through the large positively charged region on the surface of nsp2, and K111, K112, K113 were key residues. Our findings lay the foundation for a better understanding of the relationship between structure and function for nsp2. It is helpful to make full use of nsp2 as further research and development of antiviral targets and drug design.

PubMed: 34398430
DOI: 10.1007/s12250-021-00431-6

実験手法

X-RAY DIFFRACTION (1.96 Å)