7EVH

Odinarchaeota tubulin H393D mutant, in a psuedo protofilament arrangement, bound to 59% GDP, 41% phosphate

7EVH の概要

• エントリーDOI: 10.2210/pdb7evh/pdb
• 関連するPDBエントリー: 7EVB 7EVC 7EVD 7EVE 7EVG
• 分子名称: Tubulin-like protein, GUANOSINE-5'-DIPHOSPHATE, PHOSPHATE ION, ... (4 entities in total)
• 機能のキーワード: asgard, tubulin, gtp, filament, structural protein
• 由来する生物種: Odinarchaeota archaeon (strain LCB_4)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 48144.51

構造登録者

Robinson, R.C.,Akil, C.,Tran, L.T. (登録日: 2021-05-21, 公開日: 2022-03-23, 最終更新日: 2023-11-29)

主引用文献

Akil, C.,Ali, S.,Tran, L.T.,Gaillard, J.,Li, W.,Hayashida, K.,Hirose, M.,Kato, T.,Oshima, A.,Fujishima, K.,Blanchoin, L.,Narita, A.,Robinson, R.C.
Structure and dynamics of Odinarchaeota tubulin and the implications for eukaryotic microtubule evolution.
Sci Adv, 8:eabm2225-eabm2225, 2022

PubMed Abstract: Tubulins are critical for the internal organization of eukaryotic cells, and understanding their emergence is an important question in eukaryogenesis. Asgard archaea are the closest known prokaryotic relatives to eukaryotes. Here, we elucidated the apo and nucleotide-bound x-ray structures of an Asgard tubulin from hydrothermal living Odinarchaeota (OdinTubulin). The guanosine 5'-triphosphate (GTP)-bound structure resembles a microtubule protofilament, with GTP bound between subunits, coordinating the "+" end subunit through a network of water molecules and unexpectedly by two cations. A water molecule is located suitable for GTP hydrolysis. Time course crystallography and electron microscopy revealed conformational changes on GTP hydrolysis. OdinTubulin forms tubules at high temperatures, with short curved protofilaments coiling around the tubule circumference, more similar to FtsZ, rather than running parallel to its length, as in microtubules. Thus, OdinTubulin represents an evolutionary stage intermediate between prokaryotic FtsZ and eukaryotic microtubule-forming tubulins.

PubMed: 35333570
DOI: 10.1126/sciadv.abm2225

実験手法

X-RAY DIFFRACTION (2.5 Å)