7EV1

Crystal structure of LI-Cadherin EC1-2

7EV1 の概要

• エントリーDOI: 10.2210/pdb7ev1/pdb
• 関連するPDBエントリー: 7CYM
• 分子名称: Cadherin-17, CALCIUM ION, ACETATE ION, ... (5 entities in total)
• 機能のキーワード: li-cadherin, dimerization, md simulations, saxs, cell-adhesion, analytical ultracentrifugation, protein chemistry, cell adhesion
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 46716.44

構造登録者

Caaveiro, J.M.M.,Yui, A.,Tsumoto, K. (登録日: 2021-05-19, 公開日: 2021-06-16, 最終更新日: 2023-11-29)

主引用文献

Yui, A.,Caaveiro, J.M.M.,Kuroda, D.,Nakakido, M.,Nagatoishi, S.,Goda, S.,Maruno, T.,Uchiyama, S.,Tsumoto, K.
Mechanism of dimerization and structural features of human LI-cadherin.
J.Biol.Chem., 297:101054-101054, 2021

PubMed Abstract: Liver intestine (LI)-cadherin is a member of the cadherin superfamily, which encompasses a group of Ca-dependent cell-adhesion proteins. The expression of LI-cadherin is observed on various types of cells in the human body, such as normal small intestine and colon cells, and gastric cancer cells. Because its expression is not observed on normal gastric cells, LI-cadherin is a promising target for gastric cancer imaging. However, because the cell adhesion mechanism of LI-cadherin has remained unknown, rational design of therapeutic molecules targeting this cadherin has been hampered. Here, we have studied the homodimerization mechanism of LI-cadherin. We report the crystal structure of the LI-cadherin homodimer containing its first four extracellular cadherin repeats (EC1-4). The EC1-4 homodimer exhibited a unique architecture different from that of other cadherins reported so far, driven by the interactions between EC2 of one protein chain and EC4 of the second protein chain. The crystal structure also revealed that LI-cadherin possesses a noncanonical calcium ion-free linker between the EC2 and EC3 domains. Various biochemical techniques and molecular dynamics simulations were employed to elucidate the mechanism of homodimerization. We also showed that the formation of the homodimer observed in the crystal structure is necessary for LI-cadherin-dependent cell adhesion by performing cell aggregation assays. Taken together, our data provide structural insights necessary to advance the use of LI-cadherin as a target for imaging gastric cancer.

PubMed: 34364873
DOI: 10.1016/j.jbc.2021.101054

実験手法

X-RAY DIFFRACTION (1.38 Å)