7EUC

Parallel G-quadruplex structure

7EUC の概要

• エントリーDOI: 10.2210/pdb7euc/pdb
• 分子名称: DNA (5'-D(*TP*TP*GP*GP*GP*G)-3'), DNA (5'-D(P*GP*GP*GP*G)-3'), DNA (5'-D(P*GP*GP*GP*GP*T)-3'), ... (4 entities in total)
• 機能のキーワード: g-quadruplex, four-layer, parallel, l-serinol, stable, dna
• 由来する生物種: synthetic construct; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 7026.58

構造登録者

Winnerdy, F.R.,Devi, G.,Ang, J.C.Y.,Lim, K.W.,Phan, A.T. (登録日: 2021-05-17, 公開日: 2022-03-30, 最終更新日: 2024-05-15)

主引用文献

Devi, G.,Winnerdy, F.R.,Ang, J.C.Y.,Lim, K.W.,Phan, A.T.
Four-Layered Intramolecular Parallel G-Quadruplex with Non-Nucleotide Loops: An Ultra-Stable Self-Folded DNA Nano-Scaffold.
Acs Nano, 16:533-540, 2022

PubMed Abstract: A four-stranded scaffold of nucleic acids termed G-quadruplex (G4) has found growing applications in nano- and biotechnology. Propeller loops are a hallmark of the most stable intramolecular parallel-stranded G4s. To date, propeller loops have been observed to span only a maximum of three G-tetrad layers. Going beyond that would allow creation of more stable scaffolds useful for building robust nanodevices. Here we investigate the formation of propeller loops spanning more than three layers. We show that native nucleotide sequences are incompatible toward this goal, and we report on synthetic non-nucleotide linkers that form a propeller loop across four layers. With the established linkers, we constructed a four-layered intramolecular parallel-stranded G4, which exhibited ultrahigh thermal stability. Control on loop design would augment the toolbox toward engineering of G4-based nanoscaffolds for diverse applications.

PubMed: 34927423
DOI: 10.1021/acsnano.1c07630

実験手法

SOLUTION NMR