7ET4

Crystal structure of Arabidopsis TEM1 AP2 domain

7ET4 の概要

• エントリーDOI: 10.2210/pdb7et4/pdb
• 分子名称: AP2/ERF and B3 domain-containing transcription repressor TEM1, DNA (12-mer), ... (4 entities in total)
• 機能のキーワード: gene regulation, flowering time regulation, plant protein, dna binding protein, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Arabidopsis thaliana (Mouse-ear cress); 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 84751.95

構造登録者

Hu, H.,Du, J. (登録日: 2021-05-12, 公開日: 2021-09-15, 最終更新日: 2023-11-29)

主引用文献

Hu, H.,Tian, S.,Xie, G.,Liu, R.,Wang, N.,Li, S.,He, Y.,Du, J.
TEM1 combinatorially binds to FLOWERING LOCUS T and recruits a Polycomb factor to repress the floral transition in Arabidopsis.
Proc.Natl.Acad.Sci.USA, 118:-, 2021

PubMed Abstract: TEMPRANILLO 1 (TEM1) is a transcriptional repressor that participates in multiple flowering pathways and negatively regulates the juvenile-to-adult transition and the flowering transition. To understand the molecular basis for the site-specific regulation of () by TEM1, we determined the structures of the two plant-specific DNA-binding domains in TEM1, AP2 and B3, in complex with their target DNA sequences from the gene 5'-untranslated region (5'-UTR), revealing the molecular basis for TEM1 specificity for its DNA targets. In vitro binding assays revealed that the combination of the AP2 and B3 binding sites greatly enhanced the overall binding of TEM1 to the 5'-UTR, indicating TEM1 combinatorically recognizes the gene 5'-UTR. We further showed that TEM1 recruits the Polycomb repressive complex 2 (PRC2) to the 5'-UTR. The simultaneous binding of the TEM1 AP2 and B3 domains to is necessary for deposition of H3K27me3 at the 5'-UTR and for the flowering repressor function of TEM1. Overall, our data suggest that the combinatorial recognition of 5'-UTR by TEM1 ensures H3K27me3 deposition to precisely regulate the floral transition.

PubMed: 34446554
DOI: 10.1073/pnas.2103895118

実験手法

X-RAY DIFFRACTION (2.7 Å)