7ESD

Mature Donggang virus

Summary for 7ESD

• Entry DOI: 10.2210/pdb7esd/pdb
• EMDB information: 31288
• Descriptor: Genome polyprotein (2 entities in total)
• Functional Keywords: flavivirus mature, virus
• Biological source: Donggang virus; More
• Total number of polymer chains: 6
• Total formula weight: 189105.43

Authors

Zhang, Y.,Liang, D. (deposition date: 2021-05-10, release date: 2022-05-18, Last modification date: 2024-10-16)

Primary citation

Zhang, Y.,Liang, D.,Yuan, F.,Yan, Y.,Wang, Z.,Liu, P.,Yu, Q.,Zhang, X.,Wang, X.,Zheng, A.
Replication is the key barrier during the dual-host adaptation of mosquito-borne flaviviruses.
Proc.Natl.Acad.Sci.USA, 119:e2110491119-e2110491119, 2022

PubMed Abstract: Mosquito-borne flaviviruses (MBFs) adapt to a dual-host transmission circle between mosquitoes and vertebrates. Dual-host affiliated insect-specific flaviviruses (dISFs), discovered from mosquitoes, are phylogenetically similar to MBFs but do not infect vertebrates. Thus, dISF–MBF chimeras could be an ideal model to study the dual-host adaptation of MBFs. Using the pseudoinfectious reporter virus particle and reverse genetics systems, we found dISFs entered vertebrate cells as efficiently as the MBFs but failed to initiate replication. Exchange of the untranslational regions (UTRs) of Donggang virus (DONV), a dISF, with those from Zika virus (ZIKV) rescued DONV replication in vertebrate cells, and critical secondary RNA structures were further mapped. Essential UTR-binding host factors were screened for ZIKV replication in vertebrate cells, displaying different binding patterns. Therefore, our data demonstrate a post-entry cross-species transmission mechanism of MBFs, while UTR-host interaction is critical for dual-host adaptation.

PubMed: 35294288
DOI: 10.1073/pnas.2110491119

Experimental method

ELECTRON MICROSCOPY (3.9 Å)