7EQ9

Cryo-EM structure of designed protein nanoparticle TIP60 (Truncated Icosahedral Protein composed of 60-mer fusion proteins)

これはPDB形式変換不可エントリーです。

7EQ9 の概要

• エントリーDOI: 10.2210/pdb7eq9/pdb
• EMDBエントリー: 31256
• 分子名称: TIP60 (1 entity in total)
• 機能のキーワード: artificial designed protein complex, virus like particle, de novo protein
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 60
• 化学式量合計: 1067653.62

構造登録者

Obata, J.,Kawakami, N.,Tsutsumi, A.,Miyamoto, K.,Kikkawa, M.,Arai, R. (登録日: 2021-04-30, 公開日: 2021-09-15, 最終更新日: 2023-11-29)

主引用文献

Obata, J.,Kawakami, N.,Tsutsumi, A.,Nasu, E.,Miyamoto, K.,Kikkawa, M.,Arai, R.
Icosahedral 60-meric porous structure of designed supramolecular protein nanoparticle TIP60.
Chem.Commun.(Camb.), 57:10226-10229, 2021

PubMed Abstract: Supramolecular protein nanoparticles and nanocages have potential in a broad range of applications. Recently, we developed a uniform supramolecular protein nanoparticle, TIP60, symmmetrically self-assembled from fusion proteins of a pentameric Sm-like protein and a dimeric MyoX-coil domain. Herein, we report the icosahedral 60-meric structure of TIP60 solved using single-particle cryo-electron microscopy. Interestingly, the structure revealed 20 regular-triangle-like pores on the surface. TIP60 and its mutants have many modifiable sites on their exterior and interior surfaces. The TIP60 architecture will be useful in the development of biomedical and biochemical nanoparticles/nanocages for future applications.

PubMed: 34523636
DOI: 10.1039/d1cc03114g

実験手法

ELECTRON MICROSCOPY (3.3 Å)