7EPW

Crystal structure of monooxygenase Tet(X4) with tigecycline

7EPW の概要

• エントリーDOI: 10.2210/pdb7epw/pdb
• 分子名称: Flavin-dependent monooxygenase, DIHYDROFLAVINE-ADENINE DINUCLEOTIDE, TIGECYCLINE, ... (4 entities in total)
• 機能のキーワード: monooxygenase, tet(x4), tigecycline resistance, oxidoreductase
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 44719.20

構造登録者

Cheng, Q.,Chen, S. (登録日: 2021-04-28, 公開日: 2021-11-10, 最終更新日: 2023-11-29)

主引用文献

Cheng, Q.,Cheung, Y.,Liu, C.,Xiao, Q.,Sun, B.,Zhou, J.,Chan, E.W.C.,Zhang, R.,Chen, S.
Structural and mechanistic basis of the high catalytic activity of monooxygenase Tet(X4) on tigecycline.
Bmc Biol., 19:262-262, 2021

PubMed Abstract: Tigecycline is a tetracycline derivative that constitutes one of the last-resort antibiotics used clinically to treat infections caused by both multiple drug-resistant (MDR) Gram-negative and Gram-positive bacteria. Resistance to this drug is often caused by chromosome-encoding mechanisms including over-expression of efflux pumps and ribosome protection. However, a number of variants of the flavin adenine dinucleotide (FAD)-dependent monooxygenase TetX, such as Tet(X4), emerged in recent years as conferring resistance to tigecycline in strains of Enterobacteriaceae, Acinetobacter sp., Pseudomonas sp., and Empedobacter sp. To date, mechanistic details underlying the improvement of catalytic activities of new TetX enzymes are not available.

PubMed: 34895224
DOI: 10.1186/s12915-021-01199-7

実験手法

X-RAY DIFFRACTION (2.23 Å)